Skip to main content
SpringerLink
Log in

Molecular cytogenetic analysis and centromeric satellite organization of a novel 8;11 translocation in sheep: a possible intermediate in biarmed chromosome evolution

  • Published:
Mammalian Genome Aims and scope Submit manuscript

Abstract

During analysis of genome organization in sheep (Ovis aries, 2n = 54, XY/XX), we found a novel chromosomal translocation in an animal expected to be normal, adding to the six ‘centric fusions’ previously reported. The translocation was identified as t(8;11) by G-banding and was shown to be centric, involving whole chromosome arms by chromosome painting with probes for Chromosomes (Chrs) 8 and 11. Satellite I and a newly isolated satellite II clone was used to characterize the centromeric regions of both the novel and the three pairs of evolutionarily derived biarmed chromosomes. The novel t(8;11) showed satellite I proximal on both arms with satellite II covering the centromere, while the evolutionarily derived fusion leading to Chrs 2 and 3 showed the opposite configuration, not obviously derived by a simple fusion. Chr 1 has lost the satellite I hybridization patterns. The novel t(8;11) provides strong evidence for an intermediate step in evolution of the biarmed chromosomes in sheep.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5

Similar content being viewed by others

References

  1. K Anamthawat-Jonssón SE Long PK Basrur S Adalsteinsson (1992) ArticleTitleReciprocal translocation (13;20) (q12;q22) in an Icelandic sheep. Res Vet Rec 52 367–370

    Google Scholar 

  2. AN Bruere (1969) ArticleTitleMale sterility and an autosomal translocation in Romney sheep. Cytogenetics 8 209–218 Occurrence Handle1:STN:280:CCaB283ls1I%3D Occurrence Handle5816714

    CAS  PubMed  Google Scholar 

  3. AN Bruere HM Chapman DR Wyllie (1972) ArticleTitleChromosome polymorphism and its implications in the select Drysdale breed of sheep. Cytogenetics 11 233–246 Occurrence Handle1:STN:280:CSyD3cfgs1Q%3D Occurrence Handle5072786

    CAS  PubMed  Google Scholar 

  4. DJ Burkin PCM O’Brien TE Broad DF Hill CA Jones et al. (1997) ArticleTitleIsolation of chromosome-specific paints from high-resolution flow karyotypes of the sheep (Ovis aries). Chromosome Res 5 102–108 Occurrence Handle1:CAS:528:DyaK2sXjtlSrs7o%3D Occurrence Handle9146913

    CAS  PubMed  Google Scholar 

  5. R Chaves H Guedes-Pinto JS Heslop-Harrison T Schwarzacher (2000) ArticleTitleThe species and chromosomal distribution of the centromeric α-satellite I sequence from sheep in the tribe Caprini and other Bovidae. Cytogenet Cell Genet 91 62–66 Occurrence Handle1:CAS:528:DC%2BD3MXhtlals7g%3D Occurrence Handle11173832

    CAS  PubMed  Google Scholar 

  6. R Chaves F Adega S Santos JS Heslop-Harrison H Guedes-Pinto (2002) ArticleTitleIn situ hybridization and chromosome banding in mammalian species. Cytogenet Genome Res 96 113–116 Occurrence Handle1:CAS:528:DC%2BD38Xns1ejsb4%3D Occurrence Handle12438786

    CAS  PubMed  Google Scholar 

  7. L D’Aiuto P Barsanti S Mauro I Cserpan C Lavane S Ciccarese (1997) ArticleTitlePhysical relationship between satellite I and II DNA in centromeric regions of sheep chromosomes. Chromosome Res 5 375–381 Occurrence Handle1:CAS:528:DyaK2sXntVGqs7k%3D Occurrence Handle9364939

    CAS  PubMed  Google Scholar 

  8. B Glahn-Luft R Wassmuth (1978) ArticleTitleG-banding in sheep with heterozygous and homozygous translocations. Ann Génét Sél Anim 10 594

    Google Scholar 

  9. ISCNDB (2000) International system for chromosome nomenclature of domestic bovids. Di Berardino D, Di Meo GP, Gallagher DS. Hayes H, Iannuzzi L (coordinator)(eds). Cytogenet Cell Genet 92, 283–299 (2001)

  10. SRS Murthy MR Jayashankar CS Nagaraja MG Govindaiah SR Sridhara-Murthy (1995) ArticleTitleAssociation of cryptorchidism with autosomal translocation in Deccani sheep. Ind J Small Rum 1 47–48

    Google Scholar 

  11. PD Pearce HA Ansari DW Maher AA Malcolm IA Stewart-Scott et al. (1994) ArticleTitleNew Robertsonian translocation chromosomes in domestic sheep (Ovis aries). Cytogenet Cell Genet 67 137–140 Occurrence Handle1:STN:280:ByuA3MzovFA%3D Occurrence Handle8039424

    CAS  PubMed  Google Scholar 

  12. T Schwarzacher P Heslop-Harrison (2000) Practical in situ Hybridization. Bios Oxford

    Google Scholar 

  13. F Slota B Danielak A Kozubska (1986) Structural rearrangement of metacentric chromosomes in the ram of the Polish Wrzosowka breed. Abstracts of the 7th Eur Coll Cytogenet Domestic Animals Warsaw Poland 37

    Google Scholar 

  14. AT Sumner (1972) ArticleTitleA simple technique for demonstrating centromeric heterochromatin. Exp Cell Res 75 304–306 Occurrence Handle1:STN:280:CSyD2cjltFI%3D Occurrence Handle4117921

    CAS  PubMed  Google Scholar 

  15. RS Verma A Babu (1995) Human Chromosomes: Principles and Techniques. McGraw Hill New York

    Google Scholar 

Download references

Acknowledgements

This work was supported by two grants—PRAXIS XXI/BD/9046/96 and SFRH/BD/3280/2000—and a project, Molecular Cytogenetic Evolution in the Bovidae Family, POCTI/P/BIA/11285/98. We thank M.A. Ferguson-Smith and D. Burkin for the chromosome sheep paint probes. J.S. Heslop-Harrison thanks CREST, Japan Science and Technology Agency, for support of centromeric sequence work.

Author information

Authors and Affiliations

  1. Department of Genetics and Biotechnology, Centre of Genetics and Biotechnology/ICETA-UTAD, University of Trás-os-Montes and Alto Douro, P-5000-911Vila Real, Portugal

    Raquel Chaves, Filomena Adega & Henrique Guedes-Pinto

  2. Institute of Human Genetics, GSF Forschungszentrum foer Umwelt und Gesundheit and Department of Biology II, University of Munich, Richard-Wagner-Str., D-80333 Munich, Germany

    Johannes Wienberg

  3. Department of Biology, University of Leicester, Leicester LE1 7RH, UK

    John S. Heslop-Harrison

Authors
  1. Raquel Chaves
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Filomena Adega
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Johannes Wienberg
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Henrique Guedes-Pinto
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. John S. Heslop-Harrison
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Raquel Chaves.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chaves, R., Adega, F., Wienberg, J. et al. Molecular cytogenetic analysis and centromeric satellite organization of a novel 8;11 translocation in sheep: a possible intermediate in biarmed chromosome evolution . Mamm Genome 14, 706–710 (2003). https://doi.org/10.1007/s00335-003-3004-2

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00335-003-3004-2

Keywords

Search

Navigation