Abstract
Key message
Common loci and candidate genes for controlling salt-alkali tolerance and yield-related traits were identified in Brassica napus combining QTL mapping with transcriptome under salt and alkaline stresses.
Abstract
The yield of rapeseed (Brassica napus L.) is determined by multiple yield-related traits, which are susceptible to environmental factors. Many yield-related quantitative trait loci (QTLs) have been reported in Brassica napus; however, no studies have been conducted to investigate both salt-alkali tolerance and yield-related traits simultaneously. Here, specific-locus amplified fragment sequencing (SLAF-seq) technologies were utilized to map the QTLs for salt-alkali tolerance and yield-related traits. A total of 65 QTLs were identified, including 30 QTLs for salt-alkali tolerance traits and 35 QTLs for yield-related traits, accounting for 7.61–27.84% of the total phenotypic variations. Among these QTLs, 18 unique QTLs controlling two to four traits were identified by meta-analysis. Six novel and unique QTLs were detected for salt-alkali tolerance traits. By comparing these unique QTLs for salt-alkali tolerance traits with those previously reported QTLs for yield-related traits, seven co-localized chromosomal regions were identified on A09 and A10. Combining QTL mapping with transcriptome of two parents under salt and alkaline stresses, thirteen genes were identified as the candidates controlling both salt-alkali tolerance and yield. These findings provide useful information for future breeding of high-yield cultivars resistant to alkaline and salt stresses.
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Data availability
The datasets generated during the current study are available in the Sequence Read Archive (SRA) database of National Center for Biotechnology Information (NCBI) under accession PRJNA847790 and accession PRJNA847444.
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The study was supported by the key research and development projects of Yangling Seed-industry Innovation Center (Ylzy-yc2021-01).
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The research was supported by the key research and development projects of Yangling Seed-industry Innovation Center (Ylzy-yc2021-01).
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ZH was responsible for designing and supervising this study; YZ performed data analysis and manuscript drafting. YZ, QZ, HW, ST and HC worked on phenotypic survey. YS, BA and AX provided assistance for data analysis and phenotypic survey. All authors have read and approved the content of the manuscript.
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Zhang, Y., Zhang, Q., Wang, H. et al. Discovery of common loci and candidate genes for controlling salt-alkali tolerance and yield-related traits in Brassica napus L.. Plant Cell Rep 42, 1039–1057 (2023). https://doi.org/10.1007/s00299-023-03011-y
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DOI: https://doi.org/10.1007/s00299-023-03011-y