Abstract
Key message
Heterologous expression of VaMYB44 gene in Arabidopsis and V. vinifera cv. ‘Thompson Seedless’ increases cold sensitivity, which is mediated by the interaction of VaMYC2 and VaTIFY5A with VaMYB44
MYB transcription factors play critical roles in plant stress response. However, the function of MYB44 under low temperature stress is largely unknown in grapes. Here, we isolated a VaMYB44 gene from Chinese wild Vitis amurensis acc. ‘Shuangyou’ (cold-resistant). The VaMYB44 is expressed in various organs and has lower expression levels in stems and young leaves. Exposure of the cold-sensitive V. vinifera cv. ‘Thompson Seedless’ and cold-resistant ‘Shuangyou’ grapevines to cold stress (−1 °C) resulted in differential expression of MYB44 in leaves with the former reaching 14 folds of the latter after 3 h of cold stress. Moreover, the expression of VaMYB44 was induced by exogenous ethylene, abscisic acid, and methyl jasmonate in the leaves of ‘Shuangyou’. Notably, the subcellular localization assay identified VaMYB44 in the nucleus. Interestingly, heterologous expression of VaMYB44 in Arabidopsis and ‘Thompson Seedless’ grape increased freezing-induced damage compared to their wild-type counterparts. Accordingly, the transgenic lines had higher malondialdehyde content and electrolyte permeability, and lower activities of superoxide dismutase, peroxidase, and catalase. Moreover, the expression levels of some cold resistance-related genes decreased in transgenic lines. Protein interaction assays identified VaMYC2 and VaTIFY5A as VaMYB44 interacting proteins, and VaMYC2 could bind to the VaMYB44 promoter and promote its transcription. In conclusion, the study reveals VaMYB44 as the negative regulator of cold tolerance in transgenic Arabidopsis and transgenic grapes, and VaMYC2 and VaTIFY5A are involved in the cold sensitivity of plants by interacting with VaMYB44.
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Funding
This study was supported by the National Science-Technology Support Plan Projects of the Ministry of Science and Technology of the People’s Republic of China [grant number 2013BAD02B04-06] and the Shaanxi Province Key Project-Agriculture of the People’s Republic of China [Grant number 2017ZDXM-NY-026].
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ZJX designed the experiments and revised the manuscript. ZHJ performed experiments and wrote the manuscript. HYF participated in the determination of physiological-biochemical indicators. GB cloned the VaMYB44 gene. CXY participated in the revision of the manuscript. All authors approved the manuscript.
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Communicated by Kinya Toriyama.
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Fig. S1 Identification of transgenic Arabidopsis. a qRT-PCR analysis of VaMYB44 expression in transgenic Arabidopsis lines, with wild-type (WT) used as a negative control. Error bars refer to ± SE (n = 3). Significance was analyzed by the method of Least Significant Difference (LSD) (*P < 0.05; **P < 0.01) with SPSS software. b Western blot analysis of VaMYB44-GFP protein expression in transgenic lines OE1#, OE2#, and OE5#; WT was used as the control. Fig. S2 Generation and identification of transgenic ‘Thompson Seedless’ grapes. a Generation of transgenic ‘Thompson Seedless’ grapes. Unorganized callus (I); Transgenic dark-brown callus (II); Resistance screening (III); Induction of differentiation (IV); Rooting into seedlings (V); Refinement of seedlings for transplanting (VI − VII). b qRT-PCR assay for VaMYB44 overexpression in cold-resistant ‘Thompson Seedless’ grape seedlings. Error bars refer to ± SE (n = 3). Significance was analyzed by the method of Least Significant Difference (LSD) (*P < 0.05; **P < 0.01) with SPSS software. c Western blot detection of VaMYB44 overexpression in ‘Thompson Seedless’ grape seedlings. Fig. S3 Conserved domain of VaMYC2. Fig. S4 Sequence alignment of MdMYB44 and VaMYB44. Supplementary file1 (DOCX 1459 KB)
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Zhang, H., Hu, Y., Gu, B. et al. VaMYB44 transcription factor from Chinese wild Vitis amurensis negatively regulates cold tolerance in transgenic Arabidopsis thaliana and V. vinifera. Plant Cell Rep 41, 1673–1691 (2022). https://doi.org/10.1007/s00299-022-02883-w
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DOI: https://doi.org/10.1007/s00299-022-02883-w