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Glucose-6-phosphate dehydrogenase plays a pivotal role in tolerance to drought stress in soybean roots

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Abstract

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Two soybean cultivars showed markedly different drought tolerance. G6PDH plays a central role in the process of H 2 O 2 regulated GR, DHAR, and MDHAR activities to maintain GSH and Asc levels.

Abstract

Glucose-6-phosphate dehydrogenase (G6PDH) plays a pivotal role in plant resistance to environmental stresses. In this study, we investigated the role of G6PDH in modulating redox homeostasis under drought stress induced by polyethylene glycol 6000 (PEG6000) in two soybean cultivars JINDOU21 (JD-21) and WDD00172 (WDD-172). The G6PDH activity markedly increased and reached a maximum at 96 h in JD-21 and 72 h in WDD-172 during PEG6000 treatments, respectively. Glucosamine (Glucm, a G6PDH inhibitor) obviously inhibited G6PDH activity in both soybeans under PEG6000 treatments. After PEG6000 treatment, JD-21 showed higher tolerance than WDD-172 not only in higher activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), glutathione reductase (GR), dehydroascorbate reductase (DHAR), and monodehydroascorbate reductase (MDHAR), but also in higher content of glutathione (GSH) and ascorbate (Asc). And we found that hydrogen peroxide (H2O2) regulated the cell length in root elongation zone. Diphenylene iodonium (DPI, a plasma membrane NADPH oxidase inhibitor) counteracted the PEG6000-induced H2O2 accumulation and decreased the activities of GR, DHAR, and MDHAR as well as GSH and Asc content. Furthermore, exogenous application of H2O2 increased the GR, DHAR, and MDHAR activities that were decreased by Glucm under drought stress. Western blot analysis showed that the G6PDH expression was stimulated by PEG6000 and buthionine sulfoximine (BSO, glutathione biosynthesis inhibitor), and blocked by Glucm, DPI and N-acetyl-l-cysteine (NAC, GSH precursor) in both cultivars. Taken together, our evidence indicates that G6PDH plays a central role in the process of H2O2 regulated GR, DHAR, and MDHAR activities to maintain GSH and Asc levels.

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Abbreviations

Asc:

Ascorbate

DHAR:

Dehydroascorbate reductase

DPI:

Diphenylene iodonium

Glucm:

Glucosamine

G6PDH:

Glucose-6-phosphate dehydrogenase

GPX:

Glutathione peroxidase

GR:

Glutathione reductase

GSH:

Glutathione

JD-21:

JINDOU21

MDHAR:

Monodehydroaseorbate reduetase

PEG6000:

Polyethylene glycol 6000

WDD-172:

WDD00172

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Acknowledgments

We thank Tianfu Han (Institute of Crop Science, Chinese Academy of Agricultural Sciences) and Xusheng Qi (Gansu Academy of Agricultural Sciences) for WDD00172 and JINDOU21 seeds. This work was supported by the National Natural Science Foundation of China (31170225), Project of the National Eleventh-Five Year Research Program of China (2007BAC30B04), Foundation of Science and Technology Program of Gansu Province (1107RJYA005), Fundamental Research Funds for the Central Universities (lzujbky-2012-104), and Chunhuijihua Foundation (Z2008-1-62015).

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  1. Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou, 730000, People’s Republic of China

    Jie Liu, Xiaomin Wang, Yanfeng Hu, Wei Hu & Yurong Bi

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  1. Jie Liu
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  2. Xiaomin Wang
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  3. Yanfeng Hu
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  4. Wei Hu
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Correspondence to Yurong Bi.

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Communicated by K. Chong.

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Liu, J., Wang, X., Hu, Y. et al. Glucose-6-phosphate dehydrogenase plays a pivotal role in tolerance to drought stress in soybean roots. Plant Cell Rep 32, 415–429 (2013). https://doi.org/10.1007/s00299-012-1374-1

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