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Involvement of heme oxygenase-1 in β-cyclodextrin–hemin complex-induced cucumber adventitious rooting process

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Abstract

Our previous results showed that β-cyclodextrin–hemin complex (CDH) exhibited a vital protective role against cadmium-induced oxidative damage and toxicity in alfalfa seedling roots by the regulation of heme oxygenase-1 (HO-1) gene expression. In this report, we further test whether CDH exhibited the hormonal-like response. The application of CDH and an inducer of HO-1, hemin, were able to induce the up-regulation of cucumber HO-1 gene (CsHO1) expression and thereafter the promotion of adventitious rooting in cucumber explants. The effect is specific for HO-1 since the potent HO-1 inhibitor zinc protoporphyrin IX (ZnPP) blocked the above responses triggered by CDH, and the inhibitory effects were reversed further when 30 % saturation of CO aqueous solution was added together. Further, molecular evidence showed that CDH triggered the increases of the HO-1-mediated target genes responsible for adventitious rooting, including one DnaJ-like gene (CsDNAJ-1) and two calcium-dependent protein kinase (CDPK) genes (CsCDPK1 and CsCDPK5), and were inhibited by ZnPP and reversed by CO. The calcium (Ca2+) chelator ethylene glycol-bis (2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA) and the Ca2+ channel blocker lanthanum chloride (LaCl3) not only compromised the induction of adventitious rooting induced by CDH but also decreased the transcripts of above three target genes. However, the application of ascorbic acid (AsA), a well-known antioxidant in plants, failed to exhibit similar inducible effect on adventitious root formation. In short, above results illustrated that the response of CDH in the induction of cucumber adventitious rooting might be through HO-1-dependent mechanism and calcium signaling.

Key message Physiological, pharmacological and molecular evidence showed that β-cyclodextrin–hemin complex (CDH) was able to induce cucumber adventitious rooting through heme oxygenase-1 (HO-1)-dependent mechanism and calcium signaling.

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Acknowledgments

This work was supported by the Doctoral Fund of Ministry of Education of China (grant no. 20100097110019), the Natural Science Foundation of Jiangsu Province of China (grant no. BK2009309), and the Technology Support Program in Jiangsu Province, China (grant no. BE2010382).

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Authors and Affiliations

  1. College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China

    Yuting Lin, Meiyue Li & Wenbiao Shen

  2. College of Sciences, Nanjing Agricultural University, Nanjing, 210095, China

    Liqin Huang

  3. Jiangsu Key Laboratory for Supramolecular Medicinal and Applications, College of Life Science, Nanjing Normal University, Nanjing, 210097, China

    Yong Ren

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  1. Yuting Lin
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  2. Meiyue Li
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  3. Liqin Huang
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  4. Wenbiao Shen
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Corresponding author

Correspondence to Wenbiao Shen.

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Communicated by P. Kumar.

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Lin, Y., Li, M., Huang, L. et al. Involvement of heme oxygenase-1 in β-cyclodextrin–hemin complex-induced cucumber adventitious rooting process. Plant Cell Rep 31, 1563–1572 (2012). https://doi.org/10.1007/s00299-012-1270-8

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  • DOI: https://doi.org/10.1007/s00299-012-1270-8

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