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Influence of indole-butyric acid and electro-pulse on in vitro rooting and development of olive (Olea europea L.) microshoots

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Abstract

The effects of indole-butyric acid (IBA) and electro-pulses on rooting and shoot growth were studied in vitro, using olive shoot cultures. Tested shoots were obtained from seedlings belonging to three Spanish cultivars, ‘Arbequina’, ‘Manzanilla de Sevilla’ and ‘Gordal Sevillana’, which have easy-, medium- and difficult-to-root rooting abilities, respectively. The standard two-step rooting method (SRM), consisting of root induction in olive rooting medium supplemented with 0, 0.1 or 1 mg/l IBA followed by root elongation in the same rooting medium without IBA, was compared with a novel one-step method consisting of shoot electro-pulses of 250, 1,250 or 2,500 V in a solution of IBA (0, 0.1 or 1 mg/l) and direct transferral to root elongation medium. The rooting percentage of the seedling-derived shoots obtained with the SRM was 76% for ‘Arbequina’ and ‘Gordal Sevillana’ cultivars and 100% for ‘Manzanilla de Sevilla’ cultivar, whereas with the electro-pulse method, the rooting percentages were 68, 64 and 88%, respectively. IBA dipping without pulse produced 0% rooting in ‘Arbequina’ seedling-derived shoots. The electroporation in IBA not only had an effect on shoot rooting but also on shoot growth and development, with longer shoots and higher axillary shoot sprouting and growth after some of the treatments. These effects were cultivar-dependent. The electro-pulse per se could explain some of these effects on shoot development.

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Abbreviations

IBA:

Indole-3-butyric acid

NAA:

Alpha-naphthalene acetic acid

OM:

Rugini (1984) olive medium

MS:

Murashige and Skoog (1962) medium

SRM:

Standard rooting method

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Acknowledgments

We thank Dr. Lorenzo Burgos for statistical assistance and Ian Johnstone for English language editing.

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Author notes

  1. Isabel Maria Gonzalez Padilla & I. Vidoy

    Present address: IFAPA, Churriana, Málaga, Spain

Authors and Affiliations

  1. Estación Experimental La Mayora, CSIC, Algarrobo-Costa, Málaga, Spain

    Isabel Maria Gonzalez Padilla, I. Vidoy & C. L. Encina

Authors
  1. Isabel Maria Gonzalez Padilla
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  2. I. Vidoy
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  3. C. L. Encina
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Correspondence to Isabel Maria Gonzalez Padilla.

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Communicated by S. Merkle.

I.M.G. Padilla and I. Vidoy contributed equally to this article.

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Padilla, I.M.G., Vidoy, I. & Encina, C.L. Influence of indole-butyric acid and electro-pulse on in vitro rooting and development of olive (Olea europea L.) microshoots. Plant Cell Rep 28, 1411–1420 (2009). https://doi.org/10.1007/s00299-009-0740-0

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