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Regeneration of fertile green plants from oat isolated microspore culture

  • Cell Biology and Morphogenesis
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Abstract

Regeneration of fertile green plants from isolated oat microspores is reported for the first time. Factors critical for microspore growth and regeneration include cold pre-treatment, pH of culture medium and the use of conditioned culture medium. It was found that cold pre-treatment at 4°C in the dark for a minimum of 6 weeks was necessary to consistently achieve microspore growth into multicellular structures (MCS). Longer pre-treatments of up to 9 weeks were tested and found to be positively correlated with the number of MCS produced. Microspore culture medium with pH 8.0 produced significantly more MCS larger than eight cells in size than media with pH 5.8. The use of medium conditioned by actively growing barley microspores significantly increased the numbers of MCS larger than eight cells in size compared to non-conditioned media. Plants were regenerated only from cultures using conditioned medium. A total of 2 green plants and 15 albinos were regenerated. Of the green plants, one had the haploid chromosome complement (n = 3x = 21) and the other had the parental hexaploid chromosome complement (2n = 6x = 42) which may be due to spontaneous chromosome doubling. The hexaploid plant set seed naturally and the haploid plant set seed after its chromosome complement was doubled with colchicine.

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Abbreviations

AC:

Anther culture

BAP:

6-benzyleaminopurine

DH:

Doubled haploid

ELS:

Embryo-like structure

IAA:

Indole-3-acetic acid

IMC:

Isolated microspore culture

MCS:

Multicellular structure

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Acknowledgments

The authors acknowledge the excellent technical assistance of Tanya Kovalenko. This work received significant funding assistance from the South Australian Grains Industry Trust (SAGIT).

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Authors and Affiliations

  1. South Australian Research and Development Institute (SARDI), GPO Box 397, Adelaide, SA, 5001, Australia

    Parminder K. Sidhu & Philip A. Davies

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  1. Parminder K. Sidhu
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  2. Philip A. Davies
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Correspondence to Philip A. Davies.

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Communicated by R. Rose.

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Sidhu, P.K., Davies, P.A. Regeneration of fertile green plants from oat isolated microspore culture. Plant Cell Rep 28, 571–577 (2009). https://doi.org/10.1007/s00299-009-0684-4

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  • DOI: https://doi.org/10.1007/s00299-009-0684-4

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