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Production and characterization of intergeneric somatic hybrids between Brassica napus and Orychophragmus violaceus and their backcrossing progenies

  • Genetic Transformation and Hybridization
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Abstract

Alien chromosome addition lines have been widely used for identifying gene linkage groups, assigning species-specific characters to a particular chromosome and comparing gene synteny between related species. In plant breeding, their utilization lies in introgressing characters of agronomic value. The present investigation reports the production of intergeneric somatic hybrids Brassica napus (2n = 38) + Orychophragmus violaceus (2n = 24) through asymmetric fusions of mesophyll protoplasts and subsequent development of B. napus-O. violaceous chromosome addition lines. Somatic hybrids showed variations in morphology and fertility and were mixoploids (2n = 51–67) with a range of 19–28 O. violaceus chromosomes identified by genomic in situ hybridization (GISH). After pollinated with B. napus parent and following embryo rescue, 20 BC1 plants were obtained from one hybrid. These exhibited typical serrated leaves of O. violaceus or B. napus-type leaves. All BC1 plants were partially male fertile but female sterile because of abnormal ovules. These were mixoploids (2n = 41–54) with 9–16 chromosomes from O. violaceus. BC2 plants showed segregations for female fertility, leaf shape and still some chromosome variation (2n = 39–43) with 2–5 O. violaceus chromosomes, but mainly containing the whole complement from B. napus. Among the selfed progenies of BC2 plants, monosomic addition lines (2n = 39, AACC + 1O) with or without the serrated leaves of O. violaceus or female sterility were established. The complete set of additions is expected from this investigation. In addition, O. violaceus plants at diploid and tetraploid levels with some variations in morphology and chromosome numbers were regenerated from the pretreated protoplasts by iodoacetate and UV-irradiation.

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Acknowledgments

The study was supported by a grant from Education Ministry of PR China and by PCSIRT (IRT0442). We are deeply indebted to Prof. Shyam Prakash from Indian Agricultural Research Institute, New Delhi, India, for his revision of the manuscript.

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  1. Ting-ting Hu

    Present address: Xuzhou Academy of Agricultural Sciences, Xuzhou, Jiangsu, 221121, People’s Republic of China

Authors and Affiliations

  1. National Key Lab of Crop Genetic Improvement, National Center of Crop Molecular Breeding Technology, National Center of Oil Crop Improvement (Wuhan), College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Zhi-gang Zhao, Ting-ting Hu, Xian-Hong Ge, Xue-zhu Du, Li Ding & Zai-yun Li

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  1. Zhi-gang Zhao
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Correspondence to Zai-yun Li.

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Communicated by K. Toriyama.

Z. Zhao and T. Hu make equal contributions to this work.

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Zhao, Zg., Hu, Tt., Ge, XH. et al. Production and characterization of intergeneric somatic hybrids between Brassica napus and Orychophragmus violaceus and their backcrossing progenies. Plant Cell Rep 27, 1611–1621 (2008). https://doi.org/10.1007/s00299-008-0582-1

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