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Cellular redox-status is associated with regulation of frond division in Spirodela polyrrhiza

  • Cell Biology and Morphogenesis
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Abstract

We investigated a possible relationship between the levels of reactive oxygen species (ROS) and the stimulation of frond division of the aquatic plant Spirodela polyrrhiza (duckweed) during a 7-day experimental culture period. In particular, we monitored superoxide concentration using a state-of-the-art cell biosensor. A considerable reduction in ROS and superoxide concentration was observed during the first 2 days of culture, whereas duckweed cultures achieved near exponential growth rates after the second day. In addition, apoptotic markers such as the cytoplasmic concentration of cytochrome c, mitochondrial membrane depolarization and the activity of caspase-3 declined during the culture period and at least before daughter frond maturation. We suggest that S. polyrrhiza frond division may have been stimulated by the observed reduction of free radicals and the associated avoidance of cell apoptotic pathways in cultured plants.

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Acknowledgments

The authors wish to thank Dr. Federica Brandizzi (MSU-DOE Plant Research Laboratory, Michigan State University) for her valuable comments on the interpretation of the experimental results.

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Authors and Affiliations

  1. Laboratory of Plant Physiology, Faculty of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855, Athens, Greece

    Georgia Moschopoulou, Iosif Papanastasiou, Olga Makri, Katerina Soukouli & Spiridon E. Kintzios

  2. Laboratory of Enzyme Technology, Faculty of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855, Athens, Greece

    Nikos Lambrou

  3. Laboratory of Agronomy, Faculty of Plant Production, Agricultural University of Athens, Iera Odos 75, 11855, Athens, Greece

    Garyfallia Economou

Authors
  1. Georgia Moschopoulou
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  2. Iosif Papanastasiou
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  3. Olga Makri
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  4. Nikos Lambrou
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  5. Garyfallia Economou
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  6. Katerina Soukouli
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  7. Spiridon E. Kintzios
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Correspondence to Spiridon E. Kintzios.

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Communicated by F. Brandizzi.

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Moschopoulou, G., Papanastasiou, I., Makri, O. et al. Cellular redox-status is associated with regulation of frond division in Spirodela polyrrhiza . Plant Cell Rep 26, 2063–2069 (2007). https://doi.org/10.1007/s00299-007-0425-5

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  • DOI: https://doi.org/10.1007/s00299-007-0425-5

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