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Over-expression of tobacco NtHSP70-1 contributes to drought-stress tolerance in plants

  • PHYSIOLOGY AND BIOCHEMISTRY
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Abstract

HSP70, a heat shock protein, is a molecular chaperone responsive to various environmental stresses. Here, NtHSP70-1 (AY372069) was a drought-/ABA-inducible gene. We monitored the expression of CaERD15 (early responsive to dehydration, DQ267932) with exposing plants to progressive drought stress. Its activity was used as an indicator of water-deficit conditions. To analyze the protective role of HSP70, we obtained transgenic tobacco plants that constitutively expressed elevated levels of the tobacco HSP70, NtHSP70-1, as well as transgenic plants containing either the vector alone or else having NtHSP70-1 in the antisense orientation. Plants with enhanced levels of NtHSP70-1 in their transgenic sense lines exhibited tolerance to water stress. Under progressive drought, the amount of leaf NtHSP70-1 was correlated with maintenance of optimum water content, with contents being higher in the leaves of dehydrated transgenic sense plants than in those of either the control (vector-only) or the transgenic antisense plants. Moreover, the expression of CaERD15 was considerably reduced in tobacco plants that over-expressed NtHSP70-1. These results suggest that elevated levels of NtHSP70-1 can confer drought-stress tolerance.

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Abbreviations

NtHSP70-1 :

Nicotiana tabacum Heat Shock Protein 70-1

CaERD15 :

Capsicum annuum early response to dehydration gene 15

ABA:

abscisic acid

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Acknowledgements

This work was supported by a grant from the Crop Functional Genomics Center of the 21st Century Frontier Research Program (code #1434) and the Ministry of Science and Technology of the Republic of Korea to C. B. Hong. E. K. Cho was partially supported by a grant for the Pre-Doctoral Students of Korea Research Foundation.

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  1. Institute of Molecular Biology and Genetics and School of Biological Sciences, Seoul National University, Seoul, 151-742, Korea

    Eun Kyung Cho & Choo Bong Hong

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  1. Eun Kyung Cho
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  2. Choo Bong Hong
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Correspondence to Eun Kyung Cho.

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Communicated by I. S. Chung

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Cho, E.K., Hong, C.B. Over-expression of tobacco NtHSP70-1 contributes to drought-stress tolerance in plants. Plant Cell Rep 25, 349–358 (2006). https://doi.org/10.1007/s00299-005-0093-2

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  • DOI: https://doi.org/10.1007/s00299-005-0093-2

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