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Differences in the activities of some antioxidant enzymes and in H2O2 content during rhizogenesis and somatic embryogenesis in callus cultures of the ice plant

  • Physiology and Biochemistry
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Abstract

Callus was obtained from hypocotyls of Mesembryanthemum crystallinum seedlings cultured on two types of medium—germination medium (GM) and callus induction medium (CIM). Following subculture on shoot induction medium SIM1, the callus formed on CIM medium regenerated roots or somatic embryos, while that obtained on GM medium was non-regenerative. The activities of CuZn-superoxidase dismutase (SOD) were comparable in all calli, but the activities of FeSOD and MnSOD varied according to the activity of photosystem II and the regenerative potential of the tissues. Catalase (CAT) activity was related to H2O2 concentration and affected by both the culture conditions and the morphogenic potential of the calli. The possible role of CAT, SODs and H2O2 in the regeneration of M. crystallinum from callus is discussed.

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Abbreviations

BAP:

6-Benzylaminopurine

BSA:

Bovine serum albumin

CAM:

Crassulacean acid metabolism

CAT:

Catalase [EC 1.11.1.6]

DTT:

Dithiotreitol

2,4-D:

2,4-Dichlorophenoxyacetic acid

EDTA:

Ethylenediaminetetraacetic acid

F m :

Maximum chlorophyll a fluorescence

F 0 :

Minimum chlorophyll a fluorescence

Fv/Fm:

Maximum photochemical efficiency of photosystem II (Fv, the difference between Fm and F0)

NAA:

α-Naphthaleneacetic acid

NBT:

Nitro blue tetrazolium salt

PAGE:

Polyacrylamide gel electrophoresis

PEG:

Polyethylene glycol

RH:

Relative humidity

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase [EC 1.15.1.1]

TEMED:

N,N,N′,N-tetramethylethylenediamine

Tricine:

N-Tris[hydroxymethyl]methylglycine

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Acknowledgements

This study was partly supported by Polish KBN grants 6P04C 00320, 6P04F 03420, 3P04C06423 and EU grant QoL-2001-Integr to the Institute of Plant Physiology Polish Academy of Science and Deutsche Forschungsanstalt für Luft-und Raumfahrt (DLR). The Deutsche Akademische Austauschdienst (DAAD) provided the growth chamber.

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Authors and Affiliations

  1. Institute of Plant Physiology, Polish Academy of Sciences, ul. Niezapominajek 21, 30-239, Cracow, Poland

    Marta Libik, Beata Pater, Ireneusz Ślesak & Zbigniew Miszalski

  2. Department of Plant Cytology and Embryology, Jagiellonian University, ul. Grodzka 52, 31-044, Cracow, Poland

    Robert Konieczny

  3. Institute of Biology, Pedagogical Academy, ul. Podbrzezie 3, 31-054, Cracow, Poland

    Zbigniew Miszalski

Authors
  1. Marta Libik
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  2. Robert Konieczny
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  3. Beata Pater
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  4. Ireneusz Ślesak
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  5. Zbigniew Miszalski
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Corresponding author

Correspondence to Marta Libik.

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Communicated by H. Lörz

This work is dedicated to Prof. Dr. Hubert Ziegler on his 80th birthday.

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Libik, M., Konieczny, R., Pater, B. et al. Differences in the activities of some antioxidant enzymes and in H2O2 content during rhizogenesis and somatic embryogenesis in callus cultures of the ice plant. Plant Cell Rep 23, 834–841 (2005). https://doi.org/10.1007/s00299-004-0886-8

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  • DOI: https://doi.org/10.1007/s00299-004-0886-8

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