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Association of polymorphisms in TNF and GRN genes with ankylosing spondylitis in a Chinese Han population

  • Genes and Disease
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Abstract

The aim of this study is to investigate the association of the polymorphisms in tumor necrosis factor (TNF) and granulin (GRN) with ankylosing spondylitis (AS) in a Chinese Han population. Five single nucleotide polymorphisms (SNPs) covering TNF and six SNPs covering GRN were investigated in 861 Chinese Han AS patients and 864 healthy controls. For rs1799964, the C allele was linked to reduced risk of AS (p < 0.0001, OR = 0.60, 95% CI = 0.50–0.71). The carriers of the C/C homozygote showed a significantly lower risk of AS compared with the TT homozygote and the C/T heterozygote under the recessive model (p < 0.0001, OR = 0.23, 95% CI = 0.12–0.45). For rs1800629, the A allele was also linked to reduced risk of AS (p < 0.0001, OR = 0.54, 95% CI = 0.39–0.74). For rs1800630, the A allele was also linked to reduced risk of AS (p < 0.0001, OR = 0.59, 95% CI = 0.48–0.72). The carriers of the A/A homozygote showed a significantly lower risk of AS compared with the C/C homozygote and the A/C heterozygote under the recessive model (p < 0.0001, OR = 0.18, 95% CI = 0.07–0.47). For rs769178, the T allele was linked to increased risk of AS (p < 0.0001, OR = 2.59, 95% CI = 2.18–3.09). The carriers of the T/T homozygote showed a significantly higher risk of AS compared with the GG homozygote and the G/T heterozygote under the recessive model (p < 0.0001, OR = 3.34, 95 %CI = 1.95–5.72). There was no significant difference between the AS patients and the controls in the genotype or allele frequencies of rs361525. For GRN, there was no significant difference between the AS patients and the controls in the genotype or allele frequencies of rs25646, rs3760365, rs3785817, rs4792939, rs5848, rs850713 (p > 0.05). This study indicates that polymorphisms in TNF are related to AS, but polymorphisms in GRN are not related to AS susceptibility in a Chinese Han population.

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Acknowledgements

This work was supported by Foundation of Primary Research & Development Plan of Shandong Province (2015GSF118002). In the later time, we also received support from Natural Science Foundation of China Grant (81671605).

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Author notes

  1. Naiwen Hu and Yazhou Cui contributed equally to this paper.

Authors and Affiliations

  1. Department of Rheumatology and Immunology, Shandong Provincial Hospital affiliated to Shandong University, 324 Jingwu Road, Jinan, 250021, People’s Republic of China

    Naiwen Hu, Qingrui Yang, Liya Wang & Xinglin Yang

  2. Shandong Medical Biotechnological Center, Shandong Academy of Medical Sciences, Jinan, People’s Republic of China

    Yazhou Cui

  3. Department of Blood, Shandong Provincial Hospital affiliated to Shandong University, Jinan, People’s Republic of China

    Hongzhi Xu

Authors
  1. Naiwen Hu
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  2. Yazhou Cui
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  3. Qingrui Yang
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  4. Liya Wang
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  5. Xinglin Yang
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  6. Hongzhi Xu
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Contributions

Q. Yang and Y. Cui conceived and designed the study. N. Hu, X. Yang and L. Wang performed the experiments. H. Xu provided the experiment. N. Hu wrote the paper. Q. Yang and H. Xu reviewed and edited the manuscript. All authors read and approved the manuscript.

Corresponding author

Correspondence to Qingrui Yang.

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Research involving human participants and/or animals

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Informed consent was obtained from all individual participants included in the study.

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All authors declare that they have no conflict of interest.

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Hu, N., Cui, Y., Yang, Q. et al. Association of polymorphisms in TNF and GRN genes with ankylosing spondylitis in a Chinese Han population. Rheumatol Int 38, 481–487 (2018). https://doi.org/10.1007/s00296-017-3899-7

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  • DOI: https://doi.org/10.1007/s00296-017-3899-7

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