Factors affecting DNA-binding proteins and pcbC transcript levels in Penicillium chrysogenum

Abstract

Proteins extracted from Penicillium chrysogenum grown in either complex or defined medium were used in electromobility shift assays. With a probe DNA covering the region –10 to –132 relative to the pcbC translation start codon, four protein/DNA complexes (designated A–D) were reproducibly observed. Two of the four DNA/protein complexes routinely detected in extracts from liquid cultures (A and B) were also detectable with protein extracts from P. chrysogenum spores. Generally, with proteins from liquid cultures, there was a correlation between increased levels of the complexes and increased levels of the pcbC transcript. In addition, the detectable levels of the complexes and the pcbC transcript showed some relationship to the extracellular pH of the medium. In defined medium, with glucose as the carbon source, the pcbC transcript was not detectable in extracts from the wild-type strain at any time, whereas with the high penicillin-producing strain P-2, a pcbC transcript was detected. In complex medium, the pcbC transcript was detectable during the exponential phase of cultures of the wild-type or P-2, even when glucose levels were as high as 2.5% (w/v).