Skip to main content
SpringerLink
Log in

Encapsulation with HDPAF-WP of the hexane fraction of sea grape (Coccoloba uvifera L.) leaf extract by electrospraying

  • Original Paper
  • Published:
Polymer Bulletin Aims and scope Submit manuscript

Abstract

The triterpenes have low solubility, as a strategy to facility its transport into the gastro-intestinal tract, the techno-functional properties of the mixture of high degree of polymerization Agave fructans (HDPAF) and whey protein (WP) as encapsulating material for stabilized Coccoloba uvifera L. extract fraction were evaluated. The purification of the extract was carried out by column chromatography, obtaining the fraction with the highest bioactivity. The microstructure evaluated by SEM showed capsules with spherical shapes using electrospraying. HPLC–MS analysis indicated the presence of β-sitosterol and lupeol in the encapsulates. The main interactions of biopolymers and compounds were by FTIR. The Tg of the capsules (> 149 °C) and the thermal stability (> 202 °C) of the compounds were by thermal analysis (DSC and TGA, respectively). The loading and encapsulation efficiency were 100 and 95%, respectively. Finally, the encapsulates presented antioxidant capacities for ABTS, DPPH, and FIC (between 90 and 71%). The HDPAF-WP mixture has techno-functional properties that allow the stabilization of HBVC by electrospraying.

Graphical abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. WHO (World Health Organization) (2018) The top 10 causes of death. http://www.who.int/news-room/fact-sheets/detail/the-top-10-causes-of-death. Accessed date: 24 September 2020.

  2. Khoobchandani M, Bansal P, Medhe S, Ganesh N, Srivastava M M (2012) Antioxidant and antimutagenic activities of isothiocyanates rich seed oil of Eruca sativa plant. Chem Phytopotentials: Health, Energy Environ Perspect, https://doi.org/10.1007/978-3-642-23394-4_10.

  3. Santana-Gálvez J, Cisneros-Zevallos L, Jacobo-Velázquez DA (2019) A practical guide for designing effective nutraceutical combinations in the form of foods, beverages, and dietary supplements against chronic degenerative diseases. Trends Food Sci Technol 88:179–193. https://doi.org/10.1016/j.tifs.2019.03.026

    Article  CAS  Google Scholar 

  4. Noguera-Artiaga L, García-Romo JS, Rosas-Burgos EC, Cinco-Moroyoqui FJ, Vidal-Quintanar RL, Carbonell-Barrachina ÁA, Burgos-Hernández A (2019) Antioxidant, antimutagenic and cytoprotective properties of hydrosos pistachio nuts. Molecules 24:4362. https://doi.org/10.3390/molecules24234362

    Article  CAS  Google Scholar 

  5. García-Romo JS, Noguera-Artiaga L, Gálvez-Iriqui AC, Hernández-Zazueta MS, Valenzuela-Cota DF, González-Vega RI, Burgos-Hernández A (2020) Antioxidant, antihemolysis, and retinoprotective potentials of bioactive lipidic compounds from wild shrimp (Litopenaeus stylirostris) muscle. CyTA-Journal of Food 18:153–163. https://doi.org/10.1080/19476337.2020.1719210

    Article  CAS  Google Scholar 

  6. Yuan H, Ma Q, Ye L, Piao G (2016) The traditional medicine and modern medicine from natural products. Molecules 21:559. https://doi.org/10.3390/molecules21050559

    Article  CAS  Google Scholar 

  7. Calderón-Chiu C, Calderón-Santoyo M, Damasceno-Gomes S, Ragazzo-Sánchez J A (2021) Use of jackfruit leaf (Artocarpus heterophyllus L.) protein hydrolysates as a stabilizer of the nanoemulsions loaded with extract-rich in pentacyclic triterpenes obtained from Coccoloba uvifera L. leaf. Food Chem. X:100138. https://doi.org/10.1016/j.fochx.2021.100138.

  8. Lim T K (2013) Coccoloba uvifera In Edible medicinal and non-medicinal plants, Springer, Dordrecht, pp 455–45. https://doi.org/10.1007/978-94-007-5653-3_24

  9. Ramos-Hernández JA, Calderón-Santoyo M, Navarro-Ocaña A, Barros-Castillo JC, Ragazzo-Sánchez JA (2018) Use of emerging technologies in the extraction of lupeol, α-amyrin and β-amyrin from sea grape (Coccoloba uvifera L.). J Food Sci Technol 55:2377–2383. https://doi.org/10.1007/s13197-018-3152-8

    Article  CAS  Google Scholar 

  10. Ramos-Hernández JA, Calderón-Santoyo M, Burgos-Hernández A, García-Romo JS, Navarro-Ocaña A, Burboa-Zazueta MG, Sandoval-Petris E, Ragazzo-Sánchez JA (2021) Antimutagenic, antiproliferative and antioxidant properties of sea grape leaf extract fractions (Coccoloba uvifera L.). Anti-Cancer Agents Med Chem. https://doi.org/10.2174/1871520621999210104201242

    Article  Google Scholar 

  11. Ramos-Hernández JA, Ragazzo-Sánchez JA, Calderón-Santoyo M, Ortiz-Basurto RI, Prieto C, Lagaron JM (2018) Use of electrosprayed agave fructans as nanoencapsulating hydrocolloids for bioactives. Nanomaterials 8:868. https://doi.org/10.3390/nano8110868

    Article  CAS  Google Scholar 

  12. Rivera-Aguilar JO, Calderón-Santoyo M, González-Cruz EM, Ramos-Hernández JA, Ragazzo-Sánchez JA (2020) Encapsulation by electrospraying of anticancer compounds from jackfruit extract (Artocarpus heterophyllus Lam): identification, characterization and antiproliferative properties. Anti-Cancer Agents Med Chem. https://doi.org/10.2174/1871520620666200804102952

    Article  Google Scholar 

  13. González-Cruz EM, Calderón-Santoyo M, Barros-Castillo JC, Ragazzo-Sánchez JA (2021) Evaluation of biopolymers in the encapsulation by electrospraying of polyphenolic compounds extracted from blueberry (Vaccinium corymbosum L.) variety Biloxi. Polym Bull 78:3561–3576. https://doi.org/10.1007/s00289-020-03292-3

    Article  CAS  Google Scholar 

  14. Braithwaite MC, Tyagi C, Tomar LK, Kumar P, Choonara YE, Pillay V (2014) Nutraceutical-based therapeutics and formulation strategies augmenting their efficiency to complement modern medicine: an overview. J Funct Foods 6:82–99. https://doi.org/10.1016/j.jff.2013.09.022

    Article  CAS  Google Scholar 

  15. Ramos-Hernández JA, Lagarón JM, Calderón-Santoyo M, Prieto C, Ragazzo-Sánchez JA (2020) Enhancing hygroscopic stability of agave fructans capsules obtained by electrospraying. J Food Sci Technol. https://doi.org/10.1007/s13197-020-04672-3

    Article  Google Scholar 

  16. Calderón-Santoyo M, Iñiguez-Moreno M, Ragazzo-Sánchez JA (2021) Microencapsulation of citral and its antifungal activity into Pectin films. Biointerface Res Appl Chem 12:7488–7502. https://doi.org/10.33263/BRIAC126.74887502

    Article  Google Scholar 

  17. Jacobsen C, García-Moreno PJ, Mendes AC, Mateiu RV, Chronakis IS (2018) Use of electrohydrodynamic processing for encapsulation of sensitive bioactive compounds and applications in food. Annu Rev Food Sci Technol 9:525–549. https://doi.org/10.1146/annurev-food-030117-012348

    Article  CAS  Google Scholar 

  18. Prieto C, Evtoski Z, Pardo-Figuerez M, Hrakovsky J, Lagaron JM (2021) Nanostructured valsartan microparticles with enhanced bioavailability produced by high-throughput electrohydrodynamic room-temperature atomization. Mol Pharm 18:2947–2958. https://doi.org/10.1021/acs.molpharmaceut.1c00098

    Article  CAS  Google Scholar 

  19. López-Rubio A, Lagaron JM (2012) Whey protein capsules obtained through electrospraying for the encapsulation of bioactives. Innov Food Sci Emerg Technol 13:200–206. https://doi.org/10.1016/j.ifset.2011.10.012

    Article  CAS  Google Scholar 

  20. Przygodzka M, Zielińska D, Ciesarová Z, Kukurová K, Zieliński H (2014) Comparison of methods for evaluation of the antioxidant capacity and phenolic compounds in common spices. LWT-Food Sci Technol 58:321–326. https://doi.org/10.1016/j.lwt.2013.09.019

    Article  CAS  Google Scholar 

  21. Müller L, Fröhlich K, Böhm V (2011) Comparative antioxidant activities of carotenoids measured by ferric reducing antioxidant power (FRAP), ABTS bleaching assay (αTEAC), DPPH assay and peroxyl radical scavenging assay. Food Chem 129:139–148. https://doi.org/10.1016/j.foodchem.2011.04.045

    Article  CAS  Google Scholar 

  22. Wang D, Shahidi F (2018) Protein hydrolysate from turkey meat and optimization of its antioxidant potential by response surface methodology. Poult Sci 97:1824–1831. https://doi.org/10.3382/ps/pex457

    Article  CAS  Google Scholar 

  23. Burgos-Hernández A, Rosas-Burgos EC, Martínez M, Nuncio-Jauregui PN, Marhuenda F, Kačániová M, Petrová J, Carbonell-Barrachina AA (2016) Bioactive fractions from cantabrian anchovy (Engraulis encrarischolus) viscera. Food Sci Technol 36:426–431. https://doi.org/10.1590/1678-457X.10115

    Article  Google Scholar 

  24. Xia J, Yang C, Wang Y, Yang Y, Yu J (2017) Antioxidant and antiproliferative activities of the leaf extracts from Trapa bispinosa and active components. S Afr J Bot 113:377–381. https://doi.org/10.1016/j.sajb.2017.09.016

    Article  CAS  Google Scholar 

  25. Rodrigues RM, Ramos PE, Cerqueira MF, Teixeira JA, Vicente AA, Pastrana LM, Cerqueira MA (2020) Electrosprayed whey protein-based nanocapsules for β-carotene encapsulation. Food Chem 314:126157. https://doi.org/10.1016/j.foodchem.2019.126157

    Article  CAS  Google Scholar 

  26. Baskar AA, Ignacimuthu S, Paulraj GM, Al Numair KS (2010) Chemopreventive potential of β-sitosterol in experimental colon cancer model-an in vitro and in vivo study. BMC Complement Altern Med 10:24. https://doi.org/10.1186/1472-6882-10-24

    Article  CAS  Google Scholar 

  27. Siddique HR, Saleem M (2011) Beneficial health effects of lupeol triterpene: a review of preclinical studies. Life Sci 88:285–293. https://doi.org/10.1016/j.lfs.2010.11.020

    Article  CAS  Google Scholar 

  28. Agatonovic-Kustrin S, Doyle E, Gegechkori V, Morton DW (2020) High-performance thin-layer chromatography linked with (bio) assays and FTIR-ATR spectroscopy as a method for discovery and quantification of bioactive components in native Australian plants. J Pharm Biomed Anal. https://doi.org/10.1016/j.jpba.2020.113208

    Article  Google Scholar 

  29. Kutzli I, Gibis M, Baier SK, Weiss J (2019) Electrospinning of whey and soy protein mixed with maltodextrin–Influence of protein type and ratio on the production and morphology of fibers. Food Hydrocoll 93:206–214. https://doi.org/10.1016/j.foodhyd.2019.02.028

    Article  CAS  Google Scholar 

  30. Alehosseini A, Sarabi-Jamab M, Ghorani B, Kadkhodaee R (2019) Electro-encapsulation of Lactobacillus casei in high-resistant capsules of whey protein containing transglutaminase enzyme. LWT-Food Sci Technol 102:150–158. https://doi.org/10.1016/j.lwt.2018.12.022

    Article  CAS  Google Scholar 

  31. Souza ACP, Gurak PD, Marczak LDF (2017) Maltodextrin, pectin and soy protein isolate as carrier agents in the encapsulation of anthocyanins-rich extract from jaboticaba pomace. Food Bioprod Process 102:186–194. https://doi.org/10.1016/j.fbp.2016.12.012

    Article  CAS  Google Scholar 

  32. Babu S, Jayaraman S (2020) An update on β-sitosterol: a potential herbal nutraceutical for diabetic management. Biomed Pharmacother 131:110702. https://doi.org/10.1016/j.biopha.2020.110702

    Article  CAS  Google Scholar 

  33. Espinosa-Andrews H, Urias-Silvas JE (2012) Thermal properties of agave fructans (Agave tequilana Weber var. Azul). Carbohyd Polym 87:2671–2676. https://doi.org/10.1016/j.carbpol.2011.11.053

    Article  CAS  Google Scholar 

  34. Peram MR, Loveday SM, Ye A, Singh H (2013) In vitro gastric digestion of heat-induced aggregates of β-lactoglobulin. J Dairy Sci 96:63–74. https://doi.org/10.3168/jds.2012-5896

    Article  CAS  Google Scholar 

  35. Cruz-Salas CN, Prieto C, Calderón-Santoyo M, Lagarón JM, Ragazzo-Sánchez JA (2019) Micro-and nanostructures of agave fructans to stabilize compounds of high biological value via electrohydrodynamic processing. Nanomaterials 9:1659. https://doi.org/10.3390/nano9121659

    Article  CAS  Google Scholar 

  36. Jimoh FO, Adedapo AA, Afolayan AJ (2010) Comparison of the nutritional value and biological activities of the acetone, methanol and water extracts of the leaves of Solanum nigrum and Leonotis leonorus. Food Chem Toxicol 48:964–971. https://doi.org/10.1016/j.fct.2010.01.007

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors thank Tecnológico Nacional de México for their support in conducting the work throughout the project “Fracciones de extracto de hoja de uva de mar (Coccoloba uvifera L.): Evaluación de la actividad biológica y encapsulación por electroesprayado” (code 11176.21-P), Consejo Nacional de Ciencia y Tecnología (CONACyT, Mexico) for the scholarship granted (number 702624) to Jorge Alberto Ramos-Hernández; CYTED thematic network code 319RT0576 and Spanish Ministry of Science, Innovation and Universities (project code RTI2018-097249-B-C21). The author's thanks Claudia Hernández Ambrosio for technical assistance.

Funding

Tecnológico Nacional de México for their support in conducting the work throughout this project: Fracciones de extracto de hoja de uva de mar (Coccoloba uvifera L.): Evaluación de la actividad biológica y encapsulación por electroesprayado, CONACYT (Mexico) for the scholarship granted (number 467459).

Author information

Authors and Affiliations

  1. Laboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México/Instituto Tecnológico de Tepic, Av. Tecnológico 2595, C.P. 63175, Tepic, Nayarit, Mexico

    Jorge Alberto Ramos-Hernández, Montserrat Calderón-Santoyo & Juan Arturo Ragazzo-Sanchez

  2. Novel Materials and Nanotechnology Group, IATA-CSIC, Calle Catedrático Agustín Escardino Benlloch 7, 46980, Paterna, Spain

    Montserrat Calderón-Santoyo, Cristina Prieto & José Maria Lagarón

  3. Facultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Circuito Interior, Col. Copilco, C.P. 04510, Coyoacán, Ciudad de México, Mexico

    Arturo Navarro-Ocaña

Authors
  1. Jorge Alberto Ramos-Hernández
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Montserrat Calderón-Santoyo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Cristina Prieto
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. José Maria Lagarón
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Arturo Navarro-Ocaña
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Juan Arturo Ragazzo-Sanchez
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Jorge A. Ramos-Hernández: Conceptualization, Methodology, Formal analysis, Investigation, Data curation. Montserrat Calderón-Santoyo: Conceptualization, Methodology, Writing—review & editing. Cristina Prieto: Methodology, Data curation, Review & editing. José M. Lagarón: Methodology, Review & editing. Arturo Nvarro-Ocaña: Methodology, Review & editing. Juan Arturo Ragazzo-Sánchez: Conceptualization, Methodology, Project administration, Supervision, Writing—review & editing.

Corresponding author

Correspondence to Juan Arturo Ragazzo-Sanchez.

Ethics declarations

Conflict of interest

The authors declared that they have no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ramos-Hernández, J.A., Calderón-Santoyo, M., Prieto, C. et al. Encapsulation with HDPAF-WP of the hexane fraction of sea grape (Coccoloba uvifera L.) leaf extract by electrospraying. Polym. Bull. 80, 959–975 (2023). https://doi.org/10.1007/s00289-022-04088-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00289-022-04088-3

Keywords

Search

Navigation