Abstract
One of the outstanding problems in the field of heat shock response has been to elucidate the mechanism underlying the induction of heat shock proteins (HSPs). In this work, we initiate an analysis of the expression of heat shock groEL and dnaK genes and their promoters in S. pyogenes. The synthesis of total cellular proteins was studied upon transfer of a log-phase culture from 37°C to 42°C by performing 5-min pulse-labeling experiments with 35S-Met. The heat shock responses in the pathogenic Gram-positive cocci, Enterococcus faecalis and Staphylococcus aureus, were also analyzed.
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Received: 12 July 2000 / Accepted: 27 August 2000
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Laport, M., de Castro, A., Villardo, A. et al. Expression of the Major Heat Shock Proteins DnaK and GroEL in Streptococcus pyogenes: A Comparison to Enterococcus faecalis and Staphylococcus aureus . Curr Microbiol 42, 264–268 (2001). https://doi.org/10.1007/s002840110215
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DOI: https://doi.org/10.1007/s002840110215