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Agricultural Waste Management by Production of Second-Generation Bioethanol from Sugarcane Bagasse Using Indigenous Yeast Strain

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Abstract

In the wake of rapid industrialization and burgeoning transportation networks, the escalating demand for fossil fuels has accelerated the depletion of finite energy reservoirs, necessitating urgent exploration of sustainable alternatives. To address this, current research is focusing on renewable fuels like second-generation bioethanol from agricultural waste such as sugarcane bagasse. This approach not only circumvents the contentious issue of food-fuel conflicts associated with biofuels but also tackles agricultural waste management. In the present study indigenous yeast strain, Clavispora lusitaniae QG1 (MN592676), was isolated from rotten grapes to ferment xylose sugars present in the hemicellulose content of sugarcane bagasse. To liberate the xylose sugars, dilute acid pretreatment was performed. The highest reducing sugars yield was 1.2% obtained at a temperature of 121 °C for 15 min, a solid-to-liquid ratio of 1:25 (% w/v), and an acid concentration of 1% dilute acid H2SO4 that was significantly higher (P < 0.001) yield obtained under similar conditions at 100 °C for 1 h. The isolated strain was statistically optimized for fermentation process by Plackett–Burman design to achieve the highest ethanol yield. Liberated xylose sugars were completely utilized by Clavispora lusitaniae QG1 (MN592676) and gave 100% ethanol yield. This study optimizes both fermentation process and pretreatment of sugarcane bagasse to maximize bioethanol yield and demonstrates the ability of isolated strain to effectively utilize xylose as a carbon source. The desirable characteristics depicted by strain Clavispora lusitaniae shows its promising utilization in management of industrial waste like sugarcane bagasse by its conversion into renewable biofuels like bioethanol.

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Acknowledgements

The authors are thankful to the Department of Microbiology, Quaid-i-Azam University Islamabad, Pakistan for providing facilities for this research work.

Funding

This study is a part of NRPU project (20-1482NRPU/R&D/HEC/2021). The financial support to accomplish this research was provided by Higher Education Commission of Pakistan.

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Author notes

  1. Qurrat ul Ain Rana

    Present address: Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA

  2. Abdul Haq

    Present address: Peshawar Laboratories Complex, Pakistan Council of Scientific & Industrial Research, Peshawar, 25120, Pakistan

  3. Sidra Ali and Qurrat ul Ain Rana have contributed equally to this work.

Authors and Affiliations

  1. Sustainable Bioenergy and Biorefinery Laboratory, Department of Microbiology, Quaid-I-Azam University, Islamabad, 45320, Pakistan

    Sidra Ali, Qurrat ul Ain Rana, Fatima Riaz, Abdul Haq & Malik Badshah

  2. Key Laboratory of Cryospheric Science and Frozen Soil Engineering, Northwest Institute of Eco- Environment, and Resources, Chinese Academy of Sciences, Lanzhou, 730000, China

    Wasim Sajjad

  3. The Joint Bioenergy Institute, Emeryville, CA, USA

    Rahul Gauttam

  4. Department of Biological Sciences, National University of Medical Sciences, Rawalpindi, Pakistan

    Mahwish Ali

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Contributions

SA, QAR, MB conceived and designed the work. AH, WS performed statistical analysis, review and edit the manuscript. SA, QAR and FR performed the experiments and isolated strains from collected samples. SA, QAR, MB and AH acquired, analyzed, and interpreted the data. MB, AH, RG, WS,MA reviewed the manuscript, MB supervised the research work. All authors read and approved the final manuscript.

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Correspondence to Malik Badshah.

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Ali, S., Rana, Q.u.A., Riaz, F. et al. Agricultural Waste Management by Production of Second-Generation Bioethanol from Sugarcane Bagasse Using Indigenous Yeast Strain. Curr Microbiol 81, 161 (2024). https://doi.org/10.1007/s00284-024-03668-y

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