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Evaluation of Antimicrobial and Antioxidant Potential of Essential Oil from Croton piauhiensis Müll. Arg.

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Abstract

A large number of infections are caused by Gram-positive and Gram-negative multi-resistant bacteria worldwide, adding up to a figure of around 700,000 deaths per year. The indiscriminate uses of antibiotics, as well as their misuse, resulted in the selection of bacteria resistant to known antibiotics, for which it has little or no treatment. In this way, the strategies to combat the resistance of microorganisms are extremely important and, essential oils of Croton species have been extensively studied for this purpose. The aim of this study was to carry the evaluation of antibacterial, antibiofilm, antioxidant activities, and spectroscopic investigation of essential oil from Croton piauhiensis (EOCp). The EOCp exhibited antimicrobial activity against Gram-positive and Gram-negative bacteria with required MICs ranging from 0.15 to 5% (v/v). In addition, the MBC of the EOCp for Staphylococcus aureus ATCC 25923 and ATCC 700698, were 0.15 and 1.25%, respectively. Moreover, the EOCp significantly reduced significantly the biofilm production and the number of viable cells from the biofilm of all bacterial strains tested. The antioxidant potential of the EOCp showed EC50 values ranging from 171.21 to 4623.83 μg/mL. The EOCp caused hemolysis (>45%) at the higher concentrations tested (1.25 to 5%), and minor hemolysis (17.6%) at a concentration of 0.07%. In addition, docking studies indicated d-limonene as a phytochemical with potential for antimicrobial activity. This study indicated that the EOCp may be a potential agent against infections caused by bacterial biofilms, and act as a protective agent against ROS and oxidative stress.

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Acknowledgements

The authors are grateful to EMBRAPA AGROINDÚSTRIA TROPICAL for obtaining the spectral data. Edson Holanda Teixeira is Senior researcher of Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). The authors thank Editage (www.editage.com) for English language editing. The authors also thank Centro Nacional de Processamento de Alto Desempenho (CENAPAD) of the Federal University of Ceará (UFC) for providing computational resources.

Funding

This research was funded by Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico – FUNCAP (Grant: BP4-0172-00075.01.00/20 for Hélcio Silva dos Santos).

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Authors and Affiliations

  1. Laboratório Integrado de Biomoléculas (LIBS), Departamento de Patologia e Medicina Legal, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil

    Jean Parcelli Costa do Vale, Mayron Alves Vasconcelos, Francisco Vassiliepe Sousa Arruda, Nairley Cardoso Sá Firmino, Anna Luísa Pereira, Alexandre Lopes Andrade, Edson Holanda Teixeira & Hélcio Silva dos Santos

  2. Centro de Ciências Exatas e Tecnologia, Universidade Estadual Vale do Acaraú, Sobral, Ceará, Brazil

    Jean Parcelli Costa do Vale, Tigressa Helena Soares Rodrigues & Hélcio Silva dos Santos

  3. Universidade do Estado de Minas Gerais, Unidade de Divinópolis, Divinópolis, Minas Gerais, Brazil

    Mayron Alves Vasconcelos

  4. Departamento de Ciências Biológicas, Faculdade de Ciências Exatas e Naturais, Universidade do Estado do Rio Grande do Norte, Rio Grande do Norte, Mossoró, Brasil

    Mayron Alves Vasconcelos

  5. Centro Universitário Inta – Uninta, Sobral, Ceará, Brazil

    Francisco Vassiliepe Sousa Arruda & Nairley Cardoso Sá Firmino

  6. Laboratório de Produtos Naturais Marinhos, Departamento de Engenharia de Pesca, Marine Natural, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil

    Silvana Saker-Sampaio

  7. Laboratório de Biotecnologia Marinha, Departamento de Engenharia de Pesca, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil

    Alexandre Holanda Sampaio

  8. Departamento de Ciências Biológicas, Universidade Regional do Cariri, Crato, Ceará, Brazil

    Alexandre Magno Rodrigues Teixeira

  9. Programa de Pós-Graduação Ciências NaturaisCentro de Ciências E Tecnologia, Universidade Estadual Do Ceará, Fortaleza, Ceará, Brazil

    Jean Parcelli Costa do Vale & Hélcio Silva dos Santos

  10. Faculdade de Filosofia Dom Aureliano Matos, Universidade Estadual do Ceará, Limoeiro do Norte, Ceará, Brazil

    Emmanuel Silva Marinho

  11. Faculdade de Educação, Ciência E Letras de Iguatu, Universidade Estadual do Ceará, Iguatu, Ceará, Brazil

    Márcia Machado Marinho

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  1. Jean Parcelli Costa do Vale
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Contributions

Conceptualization: HSS; software: MMM; data curation, JPCV; visualization: THSR; validation: FVSA; formal analysis: ALA; Methodology: MAV; project administration: SS; funding acquisition: AMRT; writing—original draft preparation: EHT; writing—review and editing: ESM; resources:AHS; supervision: NCSF; Investigation: ALP. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Hélcio Silva dos Santos.

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do Vale, J.P.C., Vasconcelos, M.A., Arruda, F.V.S. et al. Evaluation of Antimicrobial and Antioxidant Potential of Essential Oil from Croton piauhiensis Müll. Arg.. Curr Microbiol 78, 1926–1938 (2021). https://doi.org/10.1007/s00284-021-02449-1

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