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Nakamurella albus sp. nov.: A Novel Actinobacterium Isolated from a Lichen Sample

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Abstract

A novel actinobacterium, YIM 132087T, isolated from Lepraria sp. lichen collected from Yunnan province, south-west PR China. Cells are Gram-stain-positive, catalase-positive and oxidase-negative, aerobic, non-motile and short rod-shaped. Colonies are asporogenous, circular and white brown in colour. Optimal growth occured at 15−35 °C (optimum 28 °C), at pH 5.0−9.0 (optimum pH 6.0), and in the presence of 3% NaCl (w/v). The DNA G+C content of strain YIM 132087T based on the draft genome sequence was 71.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain YIM 132087T belonged to the genus Nakamurella and exhibited high levels of 16S rRNA gene sequence similarity with Nakamurella endophytica CGMCC 4.7038T (97.9%) and Nakamurella intestinalis NBRC 111844T (97.2%). The DNA–DNA hybridization values between strain YIM 132087T and its closest relatives are lower than 26%. Strain YIM 132087T had meso-diaminopimelic acid as the diagnostic cell-wall diamino acid, and MK-8(H4) as the predominant menaquinone. Predominant cellular fatty acids (> 10%) were iso-C16:0, iso-C15:0, C16:0 and anteiso-C15:0. The polar lipid profile were found to be diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, three unknown phospholipids, one unknown aminophospholipid and one unknown lipid. Based on phenotypic, phylogenetic and chemotaxonomic analysis, strain YIM 132087T belongs to the genus Nakamurella and represents a novel species of the genus Nakamurella, for which the name Nakamurella albus sp. nov., with type strain YIM 132087T (=CGMCC 4.7629T =NBRC 114017T), is proposed.

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Acknowledgements

This research was funded by National Natural Science Foundation of China (31460005) and Major research project of Guangxi for science and technology (AA18242026).

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Authors and Affiliations

  1. Yunnan Institute of Microbiology, Key Laboratory for Conservation and Utilization of Bio-Resource, School of Life Sciences, Yunnan University, Kunming, People’s Republic of China

    Long-Qian Jiang, De-Feng An, Kun Zhang, Gui-Ding Li, Lei Lang, Cheng-Lin Jiang & Yi Jiang

  2. Institute of Microbial Pharmaceuticals, Northeastern University, Shenyang, 110819, People’s Republic of China

    Gui-Ding Li

  3. Key Lab for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, People’s Republic of China

    Xin-Yu Wang & Li-Song Wang

  4. School of Marine Science and Biotechnology, Guangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi University for Nationalities, Nanning, 530008, People’s Republic of China

    Ming-Guo Jiang

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  1. Long-Qian Jiang
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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain YIM 132087T is MN317339 and the genome sequence is WLYK00000000.

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Jiang, LQ., An, DF., Zhang, K. et al. Nakamurella albus sp. nov.: A Novel Actinobacterium Isolated from a Lichen Sample. Curr Microbiol 77, 1896–1901 (2020). https://doi.org/10.1007/s00284-020-01928-1

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