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Sequence Analysis and Characterization of Plasmid pLK39 Isolated from Endophytic Salmonella sp. Isolated from Solanum lycocarpum

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Abstract

The complete nucleotide sequence of a small cryptic plasmid pLK39 isolated from endophytic Salmonella sp. was determined. This plasmid is 4,029 bp long with an overall GC content of 55.4 %. Sequence analyses of pLK39 revealed extensive homology to several plasmids: pRK10, pK, pSW200, pBERT, pST728/06-2, pSW100, pEC3, and pUCD5000. Using the ORF Finder program, 35 putative ORFs was identified, 30 showed more than 35 residues. After performing a search for homologous sequences to the pLK39 at BLASTn software on NCBI, it was ascertained that the plasmid has a ColE1-like replication origin and also a region of mobilization proteins from relaxase family (mobCABD). Besides these mobilization proteins, the pLK39 codes a putative DUF903 protein family, which is characterized as assumed external cytoplasmic membrane lipoprotein. A recombinant form of pLK39 carrying a kanamycin resistance gene is stably maintained in Escherichia coli cells grown in the absence of selection pressure. pLK39 was compatible with pUC18, pBR322, and pACYC184.

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Acknowledgments

The authors thank Fundação de Amparo à Pesquisa do Estado de Goiás (FAPEG, edital 01/2007) for financial support.

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Authors and Affiliations

  1. Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, 74001-970, Brazil

    Pericles Lopes Dourado, Vera Lúcia Cardoso & Luiz Artur Mendes Bataus

  2. Escola de Agronomia, Universidade Federal de Goiás, Goiânia, GO, Brazil

    Alexandre Siqueira Guedes Coelho

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  1. Pericles Lopes Dourado
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  2. Vera Lúcia Cardoso
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  3. Alexandre Siqueira Guedes Coelho
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  4. Luiz Artur Mendes Bataus
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Correspondence to Luiz Artur Mendes Bataus.

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Dourado, P.L., Cardoso, V.L., Coelho, A.S.G. et al. Sequence Analysis and Characterization of Plasmid pLK39 Isolated from Endophytic Salmonella sp. Isolated from Solanum lycocarpum . Curr Microbiol 69, 846–851 (2014). https://doi.org/10.1007/s00284-014-0663-z

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  • DOI: https://doi.org/10.1007/s00284-014-0663-z

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