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Characterization of Bombyx mori Nucleopolyhedrovirus ORF109 that Encodes a 25-kDa Structural Protein of the Occlusion-Derived Virion

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Abstract

Bombyx mori nucleopolyhedrovirus (BmNPV) ORF109 (Bm109) is a gene present in many lepidopteran NPVs, but its function is unknown. In this study, Bm109 was characterized. The transcript of Bm109 was detected at 12–72 h postinfection (p.i.). Polyclonal antiserum raised to a His-BM109 fusion protein recognized BM109 in infected cell lysates from 24 to 72 h p.i., suggesting that Bm109 is a late gene. Localization of the BM109 in BV and ODV by western analyses demonstrated that BM109 was proteins of ODV.

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Acknowledgments

We thank Dr. Haifeng Shi for his support and assistance. This work was supported by the National Program of High-Tech Research and Development (863 High-Tech Program, No. 2008AA10Z145), the grants from Jiangsu Sci-Tech Support Project-Agriculture (No. BE2008379), National Basic Research Program of China (No. 2005CB121006).

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Authors and Affiliations

  1. Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang, 212013, People’s Republic of China

    Yang Zhou, Qin Yao, Hengchuan Xia, Feng Lin & Keping Chen

  2. School of Medical Science and Laboratory Medicine, Jiangsu University, 301# Xuefu Road, Zhenjiang, 212013, People’s Republic of China

    Hongxing Shen

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  1. Yang Zhou
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  2. Qin Yao
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  3. Hongxing Shen
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Correspondence to Keping Chen.

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Zhou, Y., Yao, Q., Shen, H. et al. Characterization of Bombyx mori Nucleopolyhedrovirus ORF109 that Encodes a 25-kDa Structural Protein of the Occlusion-Derived Virion. Curr Microbiol 61, 451–457 (2010). https://doi.org/10.1007/s00284-010-9637-y

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