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Induction of Plant Defense Enzymes and Phenolics by Treatment With Plant Growth–Promoting Rhizobacteria Serratia marcescens NBRI1213

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Abstract

In greenhouse experiments, plant growth–promoting rhizobacteria (PGPR) Serratia marcescens NBRI1213 was evaluated for plant growth promotion and biologic control of foot and root rot of betelvine caused by Phytophthora nicotianae. Bacterization of betelvine (Piper betle L.) cuttings with S. marcescens NBRI1213 induced phenylalanine ammonia-lyase, peroxidase, and polyphenoloxidase activities in leaf and root. Qualitative and quantitative estimation of phenolic compounds was done through high-performance liquid chromatography (HPLC) in leaf and root of betelvine after treatment with S. marcescens NBRI1213 and infection by P. nicotianae. Major phenolics detected were gallic, protocatechuic, chlorogenic, caffeic, ferulic, and ellagic acids by comparison of their retention time with standards through HPLC. In all of the treated plants, synthesis of phenolic compounds was enhanced compared with control. Maximum accumulation of phenolics was increased in S. marcescens NBRI1213–treated plants infected with P. nicotianae. In a greenhouse test, bacterization using S. marcescens NBRI1213 decreased the number of diseased plants compared with nonbacterized controls. There were significant growth increases in shoot length, shoot dry weight, root length, and root dry weight, averaging 81%, 68%, 152%, and 290%, respectively, greater than untreated controls. This is the first report of PGPR–mediated induction of phenolics for biologic control and their probable role in protecting betelvine against P. nicotianae, an important soil-borne phytopathogenic fungus.

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Acknowledgments

We are grateful to P. Pushpangadan, Director, National Botanical Research Institute (Lucknow, India) for providing the necessary facilities and encouragement. We are indebted to Dhan Prakash for analysis of the phenolic compounds by HPLC. M. Lavania is grateful to the Council of Scientific and Industrial Research (New Delhi, India) for award of the Senior Research Fellowship.

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Authors and Affiliations

  1. Division of Plant Microbe Interactions, National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226001, India

    Meeta Lavania, Puneet Singh Chauhan, Harikesh Bahadur Singh & Chandra Shekhar Nautiyal

  2. School of Life Sciences, Dr. B.R. Ambedkar University, Agra, 282002, India

    S.V.S. Chauhan

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  1. Meeta Lavania
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  2. Puneet Singh Chauhan
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  3. S.V.S. Chauhan
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  4. Harikesh Bahadur Singh
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Correspondence to Chandra Shekhar Nautiyal.

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Lavania, M., Chauhan, P.S., Chauhan, S. et al. Induction of Plant Defense Enzymes and Phenolics by Treatment With Plant Growth–Promoting Rhizobacteria Serratia marcescens NBRI1213. Curr Microbiol 52, 363–368 (2006). https://doi.org/10.1007/s00284-005-5578-2

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