Abstract
In order to quantify pathogenic epiphytic bacteria on leaf surfaces of the important European forest tree Quercus robur without time-intensive cultivation and separation of microorganisms, methods were developed to selectively quantify DNA copy numbers of the genus Erwinia in DNA isolated from the leaf surface. By using the combination of the two different real-time PCR techniques SYBR®-Green and TaqMan®, methods were developed not only to allow quantification of the total DNA copy number of Erwinia on the oak leaf surface, but also to distinguish between two significantly different groups of Erwinia strains. In the present work, these techniques were successfully applied to quantify the copy number of the genus Erwinia and its subgroups compared with the total bacteria number in DNA samples extracted from the upper leaf surface of English oaks collected on the 4th of June 2001 (Julian day 155).
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 24 June 2002 / Accepted: 25 October 2002
Rights and permissions
About this article
Cite this article
Heuser, T., Zimmer, W. Genus- and Isolate-Specific Real-Time PCR Quantification of Erwinia on Leaf Surfaces of English Oaks (Quercus robur L.). Curr Microbiol 47, 0214–0219 (2003). https://doi.org/10.1007/s00284-002-3902-7
Issue Date:
DOI: https://doi.org/10.1007/s00284-002-3902-7