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Value of whole-body dynamic 18F-FMISO PET/CT Patlak multi-parameter imaging for evaluating the early radiosensitizing effect of oleanolic acid on C6 rat gliomas

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Abstract

The purpose of this study was to investigate the value of tumour-to-muscle (T/M) ratios and Patlak Ki images extracted from whole-body dynamic 18F-fluoromisonidazole (FMISO) PET/CT Patlak multi-parameter imaging for evaluating the early radiosensitizing effect of oleanolic acid (OA). Twenty-four rats with C6 gliomas were divided into 4 groups and treated with OA (group B), radiotherapy (group C), both (group D) or neither (group A). Whole-body dynamic 18F-FMISO PET/CT scans were performed for 120 min before treatment and 24 h following the treatment course. The tumour samples were dissected for hematoxylin and eosin staining, and HIF-1α, Ki-67 and GLUT-1 immunohistochemical staining. PET images were analysed using kinetic modelling (Patlak Ki) and static analysis (T/M ratios), and correlated with immunohistochemical results. The changes in T/M ratios, Ki values and tumour volume before treatment and 24 h following the treatment course were compared, and the survival time of tumour-bearing rats was recorded. Kaplan–Meier analysis showed that OA combined with radiotherapy can inhibit tumour growth and prolong the survival time of tumour-bearing rats. Whole-body dynamic 18F-FMISO PET/CT showed that the Ki values in group D were significantly lower than those in group C, whilst there was no significant difference in T/M ratios between groups C and D. The Pearson correlation coefficient analysis showed that Ki values were significantly related to immunohistochemical results. Our study suggests that Patlak Ki images may add value to PET/CT static images for evaluating the early radio-sensitizing effect of OA.

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Data availability

The data that support the findings of this study are available on request from the corresponding author. The data are not publicly available due to privacy or ethical restrictions.

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Acknowledgements

This work was supported by grants from the National Natural Science Foundation of China (No.81971643).

Funding

National Natural Science Foundation of China, no.81971643, Huiqin Xu.

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Authors and Affiliations

  1. Department of Nuclear Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei, 230022, China

    Ke Cai, Qingqing Zhang, Hui Wang, Wenjing Yu, Yangyang Xue & Huiqin Xu

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  1. Ke Cai
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  2. Qingqing Zhang
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  3. Hui Wang
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  4. Wenjing Yu
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  5. Yangyang Xue
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Contributions

HX conceived the experiments. KC and QZ conducted the experiments. KC analysed the data and drafted the manuscript. HX, HW, WY and YX revised the paper. All authors read and approved the final manuscript.

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Correspondence to Huiqin Xu.

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The authors declare that they have no competing interests.

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All experimental animal protocols were approved by the Ethics Review Committee for Animal Experimentation of Anhui Medical University.

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Cai, K., Zhang, Q., Wang, H. et al. Value of whole-body dynamic 18F-FMISO PET/CT Patlak multi-parameter imaging for evaluating the early radiosensitizing effect of oleanolic acid on C6 rat gliomas. Cancer Chemother Pharmacol 91, 133–141 (2023). https://doi.org/10.1007/s00280-022-04502-7

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