Abstract
Objectives
Gemcitabine (Gem) is one of the most commonly used chemotherapeutic drugs in treating patients with pancreatic ductal adenocarcinoma (PDAC). Acquired drug resistance against Gem presents a major clinical challenge in the chemotherapy of PDAC. It has been shown that miRNA-3662 is lowly expressed and implicated with quantities of biological processes in cancer. However, whether miRNA-3662 regulates chemoresistance in PDAC remains largely unknown.
Materials and methods
The level of miRNA-3662 in PDAC tissues was determined by real-time qPCR (RT-qPCR). Functional experiments were used to investigate the biological role of miRNA-3662 on Gem resistance of PDAC in vitro and in vivo. Fluorescence in situ hybridization (FISH), RT-qPCR, western blotting, bioinformatics analysis and luciferase reporter assay were employed to determine the precise regulation mechanisms.
Results
In this study, it was investigated that miRNA-3662 was down-regulated in PDAC clinical samples as well as cell lines. Functional assays revealed that miRNA-3662 was sufficient to inhibit Gem resistance in PDAC cells both in vitro and in vivo. Mechanistically, hypoxia-inducible factor 1ɑ (HIF-1ɑ) was one of the transcriptional target of miRNA-3662 and was up-regulated in PDAC samples. Importantly, genetic promoting of HIF-1ɑ largely compromised miR-3662-mediated chemosensitive effects. In addition, miR-3662 could impair the aerobic glycolysis in PDAC cells.
Conclusions
This study sheds light on miRNA-3662 inhibits PDAC cell chemoresistance and aerobic glycolysis through a negative feedback loop with HIF-1ɑ. Therefore, the co-delivery of miR-3662 and Gem could be served as a promising therapeutic regimen for PDAC patients.
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Abbreviations
- Gem:
-
Gemcitabine
- PDAC:
-
Pancreatic cancer
- FISH:
-
Fluorescence in situ hybridization
- RT-qPCR:
-
Real-time qPCR
- HIF-1ɑ:
-
Hypoxia-inducible factor 1ɑ
- miRNAs:
-
MicroRNAs
- dCK:
-
Deoxycytidine kinase
- IHC:
-
Immunohistochemical
- Tunel:
-
Terminal deoxyribonucleotidyl transferase-mediated dUTP nick end labeling
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Acknowledgements
We thank the Scientific Research Fund of Hunan Provincial Education Department (No. 20A213 and No. 19B236) and Natural Science Foundation of Hunan Province (No. 2020JJ5221) for financial support.
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(I) Conception and design: all the authors; (II) administrative support: An Liu and Yonggui Zhou; (III) provision of study materials or patients: Jia Xu; (IV) collection and assembly of data: Tian Zhao and Xu Tang; (V) data analysis and interpretation: Binbin Zhou; (VI) manuscript writing: all the authors; (VII) final approval of manuscript: all the authors.
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Liu, A., Zhou, Y., Zhao, T. et al. MiRNA-3662 reverses the gemcitabine resistance in pancreatic cancer through regulating the tumor metabolism. Cancer Chemother Pharmacol 88, 343–357 (2021). https://doi.org/10.1007/s00280-021-04289-z
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DOI: https://doi.org/10.1007/s00280-021-04289-z