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Oncogenic roles of serine–threonine kinase receptor-associated protein (STRAP) in osteosarcoma

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Abstract

Purpose

To validate the presence of serine–threonine kinase receptor-associated Protein (STRAP) in osteosarcoma tissue and to investigate the oncological role of STRAP in osteosarcoma.

Methods

Expression of STRAP protein in osteosarcoma tissue compared to soft callus (hyperactive bone healing tissue) and in multiple cell lines was examined using western blot analysis. Effects of STRAP silencing on cell proliferation, invasion, migration and re-implantability in chick chorioallantoic membrane (CAM) were observed in osteosarcoma cell lines (MNNG-HOS, 143B, and U2OS).

Results

The result demonstrated that STRAP was highly up-regulated in osteosarcoma tissues compared with the normal physiological bone healing tissue (soft callus). Expression level of STRAP was markedly high in osteosarcoma cell lines with aggressive phenotype. Upon STRAP silencing, invasion and migration, but not proliferative activity, were selectively modulated in high-expression-STRAP cell lines. In addition, STRAP silencing reduced the success rate of tumor implantation and growth of MNNG-HOS cells in CAM model.

Conclusions

Serine–threonine kinase receptor-associated protein is up-regulated during osteosarcoma progression. The presence of STRAP enhances osteosarcoma cell invasion, migration and re-implantation ability, factors which play a critical role in metastasis. Serine–threonine kinase receptor-associated protein and its related pathway are worthy for further exploration as a novel target for anti-metastasis agents.

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Acknowledgements

This study was supported by Thailand Research Fund (TRF): MRG5980213; Orthopedic Laboratory and Research Network (OLARN), Faculty of Medicine, Chiang Mai University; National Research University (NRU) fund. The authors would also like to express their sincere thanks Dr. G. Lamar Robert and Assoc. Prof. Dr. Chongchit Sripun Robert for their manuscript proofreading.

Funding

This study was funded by Thailand Research Fund (TRF): MRG5980213

Author information

Authors and Affiliations

  1. Orthopedic Laboratory and Research Network (OLARN), Department of Orthopedics, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

    Dumnoensun Pruksakorn, Jeerawan Klangjorhor, Pimpisa Teeyakasem, Patsadakorn Sungngam, Parunya Chaiyawat & Areerak Phanphaisarn

  2. Biomedical Engineering Institute, Chiang Mai University, Chiang Mai, Thailand

    Dumnoensun Pruksakorn

  3. Osteology Research and Training Center (ORTC), Chiang Mai University, Chiang Mai, Thailand

    Dumnoensun Pruksakorn

  4. Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok, Thailand

    Kriengsak Lirdprapamongkol & Chantragan Srisomsap

  5. Department of Pathology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

    Jongkolnee Settakorn

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  1. Dumnoensun Pruksakorn
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Correspondence to Chantragan Srisomsap.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed (EC No. 23/2559 for Chick Chorioallantoic Membrane (CAM) assay for animal study, Chiang Mai University). All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards (OLARN Tissue Bank, Faculty of Medicine, CMU, EC-No: ID2717, Study code: ORT-2557-02717), and research protocols in this study were approved by the Ethical committee of the Faculty of Medicine, Chiang Mai University (CMU), Chiang Mai Thailand: EC No. ORT-11-09-16A-14 (ID#757).

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Informed consent was obtained from all individual participants included in the study.

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Pruksakorn, D., Klangjorhor, J., Lirdprapamongkol, K. et al. Oncogenic roles of serine–threonine kinase receptor-associated protein (STRAP) in osteosarcoma. Cancer Chemother Pharmacol 82, 1039–1047 (2018). https://doi.org/10.1007/s00280-018-3696-3

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  • DOI: https://doi.org/10.1007/s00280-018-3696-3

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