Abstract
Purpose
Τo evaluate the clinical relevance of CEACAM5mRNA-positive circulating tumor cells (CTCs) in patients with metastatic colorectal cancer (mCRC).
Methods
Peripheral blood was obtained from 436 patients with mCRC before the initiation of systemic therapy. A second sample was obtained on treatment assessment from 296 (67.9%) patients. The detection of CEACAM5mRNA-positive CTCs was performed using a real-time PCR assay.
Results
The patients’ median age was 67 years and PS (EGOG 0–1) 92%; KRAS exon 2 and BRAFV600E mutated primary tumors were identified in 31.9% and 6.4% of the tested patients, respectively, whereas metastasectomy was performed in 17.7% of the patients. Circulating CEACAM5mRNA-positive CTCs were detected in 125 (28.7%) and 85 (28.7%) patients at baseline and on treatment assessment, respectively. The detection of CEACAM5mRNA-positive cells was revealed, in multivariate analysis, as an independent prognostic factor associated with decreased PFS (HR 1.6; 95% CI 1.1–2.5; p = 0.026) and OS (HR 2.2; 95% CI 1.3–3.2; p < 0.001). The detection of CEACAM5mRNA-positive CTCs in patients with KRAS and BRAFV600E mutations was correlated with shorter PFS (p = 0.041 and p = 0.022, respectively). Moreover, OS was significantly shorter in patients with CEACAM5+/KRAS mutations compared to those with CEACAM5+/KRAS wt tumors (p = 0.023).
Conclusions
Detection of peripheral blood CEACAM5mRNA-positive CTCs is an adverse prognostic factor correlated with poor clinical outcome in patients with mCRC, especially in patients with KRAS and BRAF mutated tumors.
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Acknowledgements
This work was partly supported by the Cretan Association for Biomedical Research (CABR), the Hellenic Society of Medical Oncology (HeSMO) and the SYNERGASIA 2009 PROGRAMME (co-funded by the European Regional Development Fund and the General Secretariat of Research and Technology in Greece), Project code: Onco-Seed diagnostics.
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280_2018_3666_MOESM1_ESM.tif
Supplementary Figure 1 Kaplan Meier curves for progression-free survival (A) and overall survival (B) according to CEACAM5mRNA positivity and KRAS status at baseline and for progression-free survival (C) and overall survival (D) according to CEACAM5mRNA positivity and BRAF status at baseline (TIF 189 KB)
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Messaritakis, I., Sfakianaki, M., Papadaki, C. et al. Prognostic significance of CEACAM5mRNA-positive circulating tumor cells in patients with metastatic colorectal cancer. Cancer Chemother Pharmacol 82, 767–775 (2018). https://doi.org/10.1007/s00280-018-3666-9
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DOI: https://doi.org/10.1007/s00280-018-3666-9