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In vivo imaging of embryonic stem cell therapy

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Abstract

Embryonic stem cells (ESCs) have the most pluripotent potential of any stem cell. These cells, isolated from the inner cell mass of the blastocyst, are “pluripotent,” meaning that they can give rise to all cell types within the developing embryo. As a result, ESCs have been regarded as a leading candidate source for novel regenerative medicine therapies and have been used to derive diverse cell populations, including myocardial and endothelial cells. However, before they can be safely applied clinically, it is important to understand the in vivo behavior of ESCs and their derivatives. In vivo analysis of ESC-derived cells remains critically important to define how these cells may function in novel regenerative medicine therapies. In this review, we describe several available imaging modalities for assessing cell engraftment and discuss their strengths and limitations. We also analyze the applications of these modalities in assessing the utility of ESCs in regenerative medicine therapies.

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Acknowledgments

This work was partly sponsored by grants from the National Science Foundation of China (NSFC) (No. 30672396) and the Ministry of Science and Technology of China (No.2006DFB32940, 2011CB504400).

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Authors and Affiliations

  1. Department of Nuclear Medicine, Second Affiliated Hospital of Zhejiang University School of Medicine, 88 Jiefang Road, Hangzhou, Zhejiang, 310009, China

    Han Jiang & Hong Zhang

  2. Zhejiang University Medical PET Center, Hangzhou, China

    Han Jiang & Hong Zhang

  3. Institute of Nuclear Medicine and Molecular Imaging of Zhejiang University, Hangzhou, China

    Han Jiang & Hong Zhang

  4. Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou, China

    Han Jiang & Hong Zhang

  5. Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Bio-X Program and Stanford Cancer Center, Stanford University School of Medicine, Stanford, CA, USA

    Han Jiang & Zhen Cheng

  6. Department of Experimental Diagnostic Imaging, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA

    Mei Tian

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Jiang, H., Cheng, Z., Tian, M. et al. In vivo imaging of embryonic stem cell therapy. Eur J Nucl Med Mol Imaging 38, 774–784 (2011). https://doi.org/10.1007/s00259-010-1667-y

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