Abstract
A marine photosynthetic bacterium (PS88), identified as Rhodovulum sp., with flocculating ability was isolated from the sea sediment mud of a shrimp cultivation farm in Thailand. This bacterium flocculated in glutamate/malate medium during aerobic dark or anaerobic light cultivation. The flocculating ability was enhanced with the increase of NaCl concentration to 6% (w/v). When PS88 was grown in glutamate/malate medium containing 3.5% NaCl, protein, RNA and DNA were produced exocellularly and there was flocculation. The yields of DNA, RNA and protein were 8.3, 62.5 and 48.5 mg/g dry cell, respectively. The flocculated cells were deflocculated by treatment with a nucleolytic enzyme such as RNase or DNase, while amylase, protease, trypsin, cellulase and pectinase had no deflocculating effect. These results suggest that the exocellular nucleic acids are active in flocculation.
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Received: 10 April 1998 / Received revision: 14 July 1998 / Accepted: 8 August 1998
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Watanabe, M., Sasaki, K., Nakashimada, Y. et al. Growth and flocculation of a marine photosynthetic bacterium Rhodovulum sp.. Appl Microbiol Biotechnol 50, 682–691 (1998). https://doi.org/10.1007/s002530051351
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DOI: https://doi.org/10.1007/s002530051351