Abstract
The cefF gene of Nocardia lactamdurans, encoding a functional 2-oxoglutarate-dependent 3′-methylcephem hydroxylase (deacetoxycephalosporin C hydroxylase) has been found to be closely linked to the pcbC gene in the cephamycin C gene cluster. The open-reading frame is 933 bp long and could encode a protein of M r 34366. Introduction of cefF in the cephamycin-non-producer Streptomyces lividans conferred 3′-methylcephem-hydroxylating activity to the transformants but did not result in hydroxylation at carbon 7 of cephamycin. No 3′-methylcephem hydroxylase activity was observed when the cefF gene was introduced in S. lividans in pIJ699 (a vector containing two transcriptional terminators that prevent read-through expression), which suggests that this gene lacks an independent promoter.
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Coque, J.J.R., Enguita, F.J., Cardoza, R.E. et al. Characterization of the cefF gene of Nocardia lactamdurans encoding a 3′-methylcephem hydroxylase different from the 7-cephem hydroxylase. Appl Microbiol Biotechnol 44, 605–609 (1996). https://doi.org/10.1007/BF00172492
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DOI: https://doi.org/10.1007/BF00172492