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Fusarium verticillioides Pex7/20 mediates peroxisomal PTS2 pathway import, pathogenicity, and fumonisin B1 biosynthesis

  • Applied Genetics and Molecular Biotechnology
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Abstract

Fusarium verticillioides, a well-known fungal pathogen that causes severe disease in maize and contaminates the grains with fumonisin B1 (FB1) mycotoxin, affects the yield and quality of maize worldwide. The intrinsic roles of peroxisome targeting signal (PTS)–containing proteins in phytopathogens remain elusive. We therefore explored the regulatory role and other biological functions of the components of PTS2 receptor complex, FvPex7 and FvPex20, in F. verticillioides. We found that FvPex7 directly interacts with the carboxyl terminus of FvPex20 in F. verticillioides. PTS2-containing proteins are recognized and bound by the FvPex7 receptor or the FvPex7-Pex20 receptor complex in the cytoplasm, but the peroxisome localization of the PTS2-Pex7-Pex20 complex is only determined by Pex20 in F. verticillioides. However, we observed that some putative PTS2 proteins that interact with Pex7 are not transported into the peroxisomes, but a PTS1 protein that interacts with Pex5 was detected in the peroxisomes. Furthermore, ΔFvpex7pex20 as well as ΔFvpex7pex5 double mutants exhibited reduced pathogenicity and FB1 biosynthesis, along with defects in conidiation. The PTS2 receptor complex mutants (ΔFvpex7pex20) grew slowly on minimal media and showed reduced sensitivity to cell wall and cell membrane stress-inducing agents compared to the wild type. Taken together, we conclude that the PTS2 receptor complex mediates peroxisome matrix proteins import and contributes to pathogenicity and FB1 biosynthesis in F. verticillioides.

Key points

• FvPex7 directly interacts with FvPex20 in F. verticillioides.

• vThe PTS2 receptor complex is essential for the importation of PTS2-containing matrix protein into peroxisomes in F. verticillioides.

• Fvpex7/pex20 is involved in pathogenicity and FB1 biosynthesis in F. verticillioides.

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All data generated or analyzed during this study are included in this published article (and its supplementary information files).

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Funding

This study was funded by the National Science Foundation Program (grant number #31601599), Science and Technology Innovation Funding of FAFU (grant number #CXZX2020044A), and Open project of Fujian Provincial Key Laboratory of Crop Pest Monitoring and Control (grant number #MIMCP-202103).

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Authors and Affiliations

  1. Fujian Universities Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Mei Lin, Yakubu Saddeeq Abubakar, Jiajia Wang, Xiange Lu, Jie Zhou & Wenying Yu

  2. Department of Biochemistry, Faculty of Life Sciences, Ahmadu Bello University, Zaria, 810211, Nigeria

    Yakubu Saddeeq Abubakar

  3. Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China

    Lijing Wei, Guodong Lu, Zonghua Wang & Wenying Yu

  4. Marine Biotechnology Center, Fuzhou Institute of Oceanography, Minjiang University, Fuzhou, 350108, China

    Zonghua Wang

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  1. Mei Lin
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Guodong Lu, Zonghua Wang, Jie Zhou, and Wenying Yu conceived designed research. Mei Lin, Jiajia Wang, and Wenying Yu conducted experiments. Yakubu Saddeeq Abubakar, Lijing Wei, and Xiange Lu analyzed the data. Mei Lin, Yakubu Saddeeq Abubakar, and Wenying Yu wrote the manuscript. All authors read and approved the manuscript.

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Correspondence to Wenying Yu.

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Lin, M., Abubakar, Y.S., Wei, L. et al. Fusarium verticillioides Pex7/20 mediates peroxisomal PTS2 pathway import, pathogenicity, and fumonisin B1 biosynthesis. Appl Microbiol Biotechnol 106, 6595–6609 (2022). https://doi.org/10.1007/s00253-022-12167-8

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