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Biochemical and proteomic characterization of the extracellular enzymatic preparate of Exiguobacterium undae, suitable for efficient animal glue removal

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Abstract

In this work, we describe the preparation and characterization of a biopreparate for efficient and rapid animal glue removal. The biopreparate is based on the extracellular proteolytic enzymes of an Exiguobacterium undae environmental isolate. Liquid chromatography-mass spectrometry analysis showed that the biopreparate is predominantly composed of hydrolytic enzymes—proteases and peptidases, nucleases, peptide ABC transporter substrate-binding proteins, and a phosphatase. The two main proteins present are bacillolysin and a peptide ABC transporter substrate-binding protein. Inhibition and proteomic analyses of the biopreparate revealed that bacillolysin, a neutral metalloendopeptidase, is mainly responsible for its proteolytic activity. This biopreparate was able to satisfactorily remove two types of animal glue from different kinds of material surfaces. These results suggest that this biopreparate could serve as a potential new tool for the restoration of historical objects rather than living microorganisms.

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Acknowledgements

The authors thank Dr. Zdenek Voburka (Institute of Organic Chemistry and Biochemistry Czech Academy of Sciences, Prague, Czech Republic) for N-terminal sequencing and Dr. Jacob A. Bauer for discussion and manuscript revision.

Funding

The work was financially supported by the grant APVV-15-0528 “Modified polymers from renewable resources and their degradation.” This contribution is also the result of the project ITMS-26240220010 in the frame of the support program Research and Development of the European Regional Development Fund. A Slovak patent application form, No. PP50012-2018, has been applied for the E. undae biopreparate.

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Authors and Affiliations

  1. Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, 845 51, Bratislava, Slovakia

    Lenka Jeszeová, Vladena Bauerová-Hlinková, Andrea Puškárová, Mária Bučková, Lucia Kraková & Domenico Pangallo

  2. Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38, Bratislava, Slovakia

    Peter Baráth

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  1. Lenka Jeszeová
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  2. Vladena Bauerová-Hlinková
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  4. Andrea Puškárová
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  5. Mária Bučková
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  6. Lucia Kraková
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  7. Domenico Pangallo
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Correspondence to Vladena Bauerová-Hlinková or Domenico Pangallo.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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The authors declare that they have no conflict of interest.

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Jeszeová, L., Bauerová-Hlinková, V., Baráth, P. et al. Biochemical and proteomic characterization of the extracellular enzymatic preparate of Exiguobacterium undae, suitable for efficient animal glue removal. Appl Microbiol Biotechnol 102, 6525–6536 (2018). https://doi.org/10.1007/s00253-018-9105-3

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  • DOI: https://doi.org/10.1007/s00253-018-9105-3

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