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Characterization of the biochemical, physiological, and medicinal properties of Streptomyces hygroscopicus ACTMS-9H isolated from the Amazon (Brazil)

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Abstract

Actinomycetes are known to produce numerous secondary bioactive metabolites of pharmaceutical interest. The purpose of this study was to isolate, characterize, and investigate the antibacterial, antifungal, and anticancer activities of metabolites produced by Actinobacteria isolated from the rhizosphere of Paullinia cupana. The Actinobacteria was identified as Streptomyces hygroscopicus ACTMS-9H. Based on a bioguided study, the methanolic biomass extract obtained from submerged cultivation had the most potent antibacterial, antifungal, and cytotoxic activities. This extract was partitioned with n-hexane, ethyl acetate, and 2-butanol. Elaiophylin was isolated from the methanolic biomass extract, and its molecular formula was determined (C54H88O18) based on 1H and 13C NMR, IR and MS analyses. The 2-butanol phase was fractionated into four fractions (EB1, EB2A, EB2B, and EB3M). Chemical prospecting indicated the presence of alkaloids, saponins, and reducing sugars in the methanolic extract and 2-butanol phase. The elaiophylin displayed anticancer activity in HEp-2 and HL-60 cells with an IC50 of 1 μg/mL. The EB1 fraction was selectively toxic to HL-60 cells with IC50 of 9 ng/mL. Bioautography showed that the EB1 fraction contained an alkaloid with antibacterial and antifungal activities (MIC values ≤1.9 and <3.9 μg/mL, respectively). In conclusion, the EB1 fraction and elaiophylin of S. hygroscopicus have potent antimicrobial, antifungal, and anticancer activities.

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Acknowledgments

The authors thank the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Fundação de Amparo a Ciência e Tecnologia de Pernambuco (FACEPE) for financial support. The authors thank CETENE for the use of the Scanning Electron Microscopy. We are grateful to Maria D. Rodrigues for the technical assistance. This manuscript was edited for the English language by American Journal Experts (certificate verification key: 93F3-2B7F-4692-D4CA-B62F).

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Authors and Affiliations

  1. Departamento de Antibióticos, Rua Prof. Moraes Rego, 1235, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil

    Sandrine M. A. Lima, Janaína. G. S. Melo, Gláucia M. S. Lima, Maria do Carmo A. Lima, Jaciana S. Aguiar, Janete M. Araújo & Teresinha G. Silva

  2. Departamento de Fisiologia e Farmacologia, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil

    Gardênia C. G. Militão

  3. Instituto de Química, Universidade Federal do Rio Grande do Norte, Lagoa Nova, Rio Grande do Norte, 59.072-970, Brazil

    Renata M. Araújo

  4. Laboratório de Ciências Químicas, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, Rio de Janeiro, 28013-602, Brazil

    Raimundo Braz-Filho

  5. Departamento de Química, Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro, 23890-000, Brazil

    Raimundo Braz-Filho

  6. Laboratoire de Chimie Thérapeutique, Cibles et Médicaments des Infections et du Cancer, IICiMed EA 1155, Institut de Recherche en Santé 2, Université de Nantes, 22 Boulevard Bénoni Goullin, 44200, Nantes, France

    Pascal Marchand

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  1. Sandrine M. A. Lima
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Correspondence to Teresinha G. Silva.

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Lima, S.M.A., Melo, J.G.S., Militão, G.C.G. et al. Characterization of the biochemical, physiological, and medicinal properties of Streptomyces hygroscopicus ACTMS-9H isolated from the Amazon (Brazil). Appl Microbiol Biotechnol 101, 711–723 (2017). https://doi.org/10.1007/s00253-016-7886-9

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