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s-triazine degrading bacterial isolate Arthrobacter sp. AK-YN10, a candidate for bioaugmentation of atrazine contaminated soil

  • Environmental biotechnology
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Abstract

The Arthrobacter sp. strain AK-YN10 is an s-triazine pesticide degrading bacterium isolated from a sugarcane field in Central India with history of repeated atrazine use. AK-YN10 was shown to degrade 99 % of atrazine in 30 h from media supplemented with 1000 mg L−1 of the herbicide. Draft genome sequencing revealed similarity to pAO1, TC1, and TC2 catabolic plasmids of the Arthrobacter taxon. Plasmid profiling analyses revealed the presence of four catabolic plasmids. The trzN, atzB, and atzC atrazine-degrading genes were located on a plasmid of approximately 113 kb.

The flagellar operon found in the AK-YN10 draft genome suggests motility, an interesting trait for a bioremediation agent, and was homologous to that of Arthrobacter chlorophenolicus. The multiple s-triazines degradation property of this isolate makes it a good candidate for bioremediation of soils contaminated by s-triazine pesticides.

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Acknowledgments

Funds from the Department of Biotechnology, New Delhi are gratefully acknowledged. Author Sneha Sagarkar acknowledges the Raman Charpak funding to work in the laboratory of Agroecology at INRA of Dijon (France). Pooja Bhardwaj is grateful to CSIR/UGC (India) for a senior research fellowship award.

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Authors and Affiliations

  1. Environmental Genomics Division, National Environmental Engineering Research Institute (CSIR-NEERI), Nehru Marg, Nagpur, 440 020, India

    Sneha Sagarkar, Pooja Bhardwaj & Atya Kapley

  2. INRA, UMR 1347 Agroécologie, 17 rue Sully, B.P. 86510, 21065, Dijon Cedex, France

    Veronika Storck, Marion Devers-Lamrani & Fabrice Martin-Laurent

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  1. Sneha Sagarkar
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  2. Pooja Bhardwaj
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  3. Veronika Storck
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  4. Marion Devers-Lamrani
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  5. Fabrice Martin-Laurent
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  6. Atya Kapley
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Correspondence to Atya Kapley.

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Sagarkar, S., Bhardwaj, P., Storck, V. et al. s-triazine degrading bacterial isolate Arthrobacter sp. AK-YN10, a candidate for bioaugmentation of atrazine contaminated soil. Appl Microbiol Biotechnol 100, 903–913 (2016). https://doi.org/10.1007/s00253-015-6975-5

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  • DOI: https://doi.org/10.1007/s00253-015-6975-5

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