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Influence of the pH on the itaconic acid production with Aspergillus terreus

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Abstract

Itaconic acid is mainly produced with the filamentous fungi Aspergillus terreus. An increase in the pH during the production phase of the cultivation resulted in an increase in the itaconic acid concentration. The pH was raised by a single pH shift ranging from pH 4 to 6 or by a pH control to pH 3. Different lyes can be used for the pH shift, but ammonia solution has proven to be the best, because here the productivity does not drop after the pH shift. The highest itaconic acid concentration of 146 g/L was reached when a pH control to pH 3 was started after 2.1 days of cultivation. This is an increase of 68 % to the cultivation without pH control. When this technique was combined with previously found optimizations, a final itaconic acid concentration of 129 g/L was reached after 4.7 days of cultivation, resulting in a productivity of 1.15 g/L/h.

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References

  • Batti M, Schweiger LB (1963) Process for the production of itaconic acid. US patent 3,078,217 (to Miles Laboratories)

  • Caddick M, Brownlee A, Arst H Jr (1986) Regulation of gene expression by pH of the growth medium in Aspergillus nidulans. Mol Gen Genet 203(2):346–353

    Article  CAS  PubMed  Google Scholar 

  • Cleland W, Johnson MJ (1956) Studies on the formation of oxalic acid by Aspergillus niger. J Biol Chem 220(2):595–606

    CAS  PubMed  Google Scholar 

  • Gyamerah MH (1995) Oxygen requirement and energy relations of itaconic acid fermentation by Aspergillus terreus NRRL 1960. Appl Microbiol Biotechnol 44(1–2):20–26

    Article  Google Scholar 

  • Hevekerl A, Kuenz A, Vorlop KD (2014) Filamentous fungi in microtiter plates—an easy way to optimize itaconic acid production with Aspergillus terreus. Appl Microbiol Biotechnol 98(16):6983–6989

    Article  CAS  PubMed  Google Scholar 

  • Jaklitsch WM, Kubicek CP, Scrutton MC (1991) The subcellular organisation of itaconate biosynthesis in Aspergillus terreus. J Gen Microbiol 137(3):533–539

  • Kautola H (1990) Itaconic acid production from xylose in repeated-batch and continuous bioreactors. Appl Microbiol Biotechnol 33(1):7–11

    Article  CAS  Google Scholar 

  • Kinoshita K (1932) Über die Produktion von Itaconsäure und Mannit durch einen neuen Schimmelpilz. Aspergillus itaconicus. Acta Phytochimica 5:271–287

    Google Scholar 

  • Kuenz A, Gallenmüller Y, Willke T, Vorlop K-D (2012) Microbial production of itaconic acid: developing a stable platform for high product concentrations. Appl Microbiol Biotechnol 96(5):1209–1216

    Article  CAS  PubMed  Google Scholar 

  • Larsen H, Eimhjellen K (1955) The mechanism of itaconic acid formation by Aspergillus terreus. 1. The effect of acidity. Biochem J 60(1):135

    CAS  PubMed Central  PubMed  Google Scholar 

  • Lockwood LB, Reeves MD (1945) Some factors affecting the production of itaconic acid by Aspergillus terreus. Arch Biochem 6(3):455–469

    CAS  Google Scholar 

  • Mischak H, Kubicek CP, Röhr M (1985) Formation and location of glucose oxidase in citric acid producing mycelia of Aspergillus niger. Appl Microbiol Biotechnol 21(1–2):27–31

    CAS  Google Scholar 

  • Okabe M, Lies D, Kanamasa S, Park EY (2009) Biotechnological production of itaconic acid and its biosynthesis in Aspergillus terreus. Appl Microbiol Biotechnol 84(4):597–606

    Article  CAS  PubMed  Google Scholar 

  • Park YS, Ohta N, Okabe M (1993) Effect of dissolved oxygen concentration and impeller tip speed on itaconic acid production by Aspergillus terreus. Biotechnol Lett 15(6):583–586

    Article  CAS  Google Scholar 

  • Riscaldati E, Moresi M, Federici F, Petruccioli M (2000) Effect of pH and stirring rate on itaconate production by Aspergillus terreus. J Biotechnol 83(3):219–230

    Article  CAS  PubMed  Google Scholar 

  • Rychtera M, Wase DAJ (1981) The growth of Aspergillus terreus and the production of itaconic acid in batch and continuous cultures. The influence of pH. J Chem Technol Biotechnol 31(8):509–521

    Article  CAS  Google Scholar 

  • Soccol CR, Vandenberghe LP, Rodrigues C, Pandey A (2006) New perspectives for citric acid production and application. Food Technol Biotechnol 44(2):141–149

    CAS  Google Scholar 

  • Tate BE (1981) Itaconic acid and derivatives. Grayson M Eckroth E (eds) Kirk-Othmer Encycl Chem Technol 3:865–873

  • Willke T, Vorlop K-D (2001) Biotechnological production of itaconic acid. Appl Microbiol Biotechnol 56(3–4):289–295

    Article  CAS  PubMed  Google Scholar 

  • Yahiro K, Takahama T, Park YS, Okabe M (1995) Breeding of Aspergillus terreus mutant TN-484 for itaconic acid production with high yield. J Ferment Bioeng 79(5):506–508

    Article  CAS  Google Scholar 

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Acknowledgments

Many thanks for the financial support by the Fachagentur Nachwachsende Rohstoffe e.V. (grant no. 22020908).

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Authors and Affiliations

  1. Thünen-Institute of Agricultural Technology, Bundesallee 50, 38116, Braunschweig, Germany

    Antje Hevekerl, Anja Kuenz & Klaus-Dieter Vorlop

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  1. Antje Hevekerl
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  2. Anja Kuenz
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  3. Klaus-Dieter Vorlop
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Correspondence to Anja Kuenz.

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Hevekerl, A., Kuenz, A. & Vorlop, KD. Influence of the pH on the itaconic acid production with Aspergillus terreus . Appl Microbiol Biotechnol 98, 10005–10012 (2014). https://doi.org/10.1007/s00253-014-6047-2

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  • DOI: https://doi.org/10.1007/s00253-014-6047-2

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