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Caecal environment of rats fed far East Asian-modelled diets

  • Environmental biotechnology
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Abstract

To clarify the effect of type of foods on the intestinal environment, Far East Asian- (FEA; rich in rice starch, soy protein and soy oil) and Far East Asian marine- (FEAM; rich in rice starch, fish meal, fish oil and brown alga) modelled diets and sucrose, casein and beef tallow-rich (SCB) diet were prepared. After the 2-week administration of diets in rats, caecal organic acids and putrefactive compounds (ammonia, indole, phenol and H2S, which are regarded as putative risk factors for tumours) were determined. The caecal microbiota was also analyzed using denaturing gradient gel electrophoresis and pyrosequencing with bar-coded primers targeting the bacterial 16S rRNA gene. Levels of n-butyrate, acetate, indole and phenol were high in rats fed FEA. On the other hand, H2S was clearly suppressed by both FEA and FEAM comparing with SCB. These results suggest that FEAM is preferable to FEA for optimal intestinal environment and host health. Both microbial analyses showed that the diversity of microbiota in the FEAM group was lower than in the other diet groups. Ratio of Firmicutes, Bacteroidetes and Proteobacteria in the SCB group was about 5:4:1. Firmicutes, particularly Lachnospiraceae, was promoted by FEA and FEAM.

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Acknowledgments

The present study was supported in part by the Ministry of Education, Science, Sports and Culture, Japan, Grant-in-Aid for Scientific Research (C), #25450300, 2013–2015 and was also supported by a 2013 Grant from Fuji Foundation for Protein Research (Osaka, Japan).

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  1. Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Konan, Minato-ku, Tokyo, 108-8477, Japan

    Choa An, Takashi Kuda, Takahiro Yazaki, Hajime Takahashi & Bon Kimura

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  1. Choa An
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  2. Takashi Kuda
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  3. Takahiro Yazaki
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  4. Hajime Takahashi
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  5. Bon Kimura
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Correspondence to Takashi Kuda.

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An, C., Kuda, T., Yazaki, T. et al. Caecal environment of rats fed far East Asian-modelled diets. Appl Microbiol Biotechnol 98, 4701–4709 (2014). https://doi.org/10.1007/s00253-014-5535-8

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