Abstract
Deinococcus geothermalis metabolism has been scarcely studied to date, although new developments on its utilization for bioremediation have been carried out. So, large-scale production of this strain and a better understanding of its physiology are required. A fed-batch experiment was conducted to achieve a high cell density non-limiting culture of D. geothermalis DSM 11302. A co-substrate nutritional strategy using glucose and yeast extract was carried out in a 20-L bioreactor in order to maintain a non-limited growth at a maximal growth rate of 1 h−1 at 45 °C. Substrate supplies were adjusted by monitoring online culture parameters and physiological data (dissolved oxygen, gas analyses, respiratory quotient, biomass concentration). The results showed that yeast extract could serve as both carbon and nitrogen sources, although glucose and ammonia were consumed too. Yeast extract carbon-specific uptake rate reached a value 4.5 times higher than glucose carbon-specific uptake rate. Cell concentration of 9.6 g L−1 dry cell weight corresponding to 99 g of biomass was obtained using glucose and yeast extract as carbon and nitrogen sources.
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References
Battista JR (1997) Against all odds: the survival strategies of Deinococcus radiodurans. Annu Rev Microbiol 51:203–224
Battista JR, Earl AM, Park MJ (1999) Why is Deinococcus radiodurans so resistant to ionizing radiation? Trends Microbiol 7:362–365
Blasius M, Sommer S, Hübscher U (2008) Deinococcus radiodurans: what belongs to the survival kit? Crit Rev Biochem Mol Biol 43:221–238
Brim H, Venkateswaran A, Kostandarithes HM, Fredrickson JK, Daly MJ (2003) Engineering Deinococcus geothermalis for bioremediation of high-temperature radioactive waste environments. Appl Environ Microbiol 69:4575–4582
Cox MM, Battista JR (2005) Deinococcus radiodurans—the consummate survivor. Nat Rev Microbiol 3:882–892
Daly MJ (2006) Modulating radiation resistance: insights based on defenses against reactive oxygen species in the radioresistant bacterium Deinococcus radiodurans. Clin Lab Med 26:491–505
Daly MJ (2009) A new perspective on radiation resistance based on Deinococcus radiodurans. Nat Rev Microbiol 7:237–245
Daly MJ, Gaidamakova EK, Matrosova VY, Vasilenko A, Zhai M, Venkateswaran A, Hess M, Omelchenko MV, Kostandarithes HM, Makarova KS, Wackett LP, Fredrickson JK, Ghosal D (2004) Accumulation of Mn(II) in Deinococcus radiodurans facilitates gamma-radiation resistance. Science 306:1025–1028
Egli T, Fiechter A (1981) Theoretical analysis of media used in the growth of yeasts on methanol. J Gen Microbiol 123:365–369
Ferreira AC, Nobre MF, Rainey FA, Silva MT, Wait R, Burghardt J, Chung AP, DaCosta MS (1997) Deinococcus geothermalis sp. nov. and Deinococcus murrayi sp. nov., two extremely radiation-resistant and slightly thermophilic species from hot springs. Int J Syst Bacteriol 47:939–947
Gornall AG, Bardawill CJ, David MM (1949) Determination of serum proteins by means of the Biuret reaction. J Biol Chem 177:751–766
He Y (2009) High cell density production of Deinococcus radiodurans under optimized conditions. J Ind Microbiol Biotechnol 36:539–546
Holland A, Rothfuss H, Lidstrom M (2006) Development of a defined medium supporting rapid growth for Deinococcus radiodurans and analysis of metabolic capacities. Appl Microbiol Biotechnol 72:1074–1082
Holt JG, Bergey DH (1994) Gram-positive cocci. In: Hensyl WR (ed) Bergey’s manual of determinative bacteriology. Williams & Wilkins, Baltimore, p 527
Kongpol A, Kato J, Vangnai AS (2008) Isolation and characterization of Deinococcus geothermalis T27, a slightly thermophilic and organic solvent-tolerant bacterium able to survive in the presence of high concentrations of ethyl acetate. FEMS Microbiol Lett 286:227–235
Korz DJ, Rinas U, Hellmuth K, Sanders EA, Deckwer WD (1995) Simple fed-batch technique for high cell density cultivation of Escherichia coli. J Biotechnol 39:59–65
Lee J, Lee SY, Park S, Middelberg AP (1999) Control of fed-batch fermentations. Biotechnol Adv 17:29–48
Liedert C, Peltola M, Bernhardt J, Neubauer P, Salkinoja-Salonen M (2012) Physiology of resistant Deinococcus geothermalis bacterium aerobically cultivated in low-manganese medium. J Bacteriol 194:1552–1561
Makarova KS, Omelchenko MV, Gaidamakova EK, Matrosova VY, Vasilenko A, Zhai M, Lapidus A, Copeland A, Kim E, Land M, Mavrommatis K, Pitluck S, Richardson PM, Detter C, Brettin T, Saunders E, Lai B, Ravel B, Kemner KM, Wolf YI, Sorokin A, Gerasimova AV, Gelfand MS, Fredrickson JK, Koonin EV, Daly MJ (2007) Deinococcus geothermalis: the pool of extreme radiation resistance genes shrinks. PLoS One 2:e955
Mattimore V, Battista JR (1996) Radioresistance of Deinococcus radiodurans: functions necessary to survive ionizing radiation are also necessary to survive prolonged desiccation. J Bacteriol 178:633–637
Murray RG, Hall M, Thompson BG (1983) Cell division in Deinococcus radiodurans and a method for displaying septa. Can J Microbiol 29:1412–1423
Riesenberg D, Guthke R (1999) High-cell-density cultivation of microorganisms. Appl Microbiol Biotechnol 51:422–430
Roels JA (1983) Energetics and kinetics in biotechnology. Elsevier, Amsterdam
Schneebeli R, Egli T (2013) A defined, glucose-limited mineral medium for the cultivation of Listeria spp. Appl Environ Microbiol 79:2503–2511
Slade D, Radman M (2011) Oxidative stress resistance in Deinococcus radiodurans. Microbiol Mol Biol Rev 75:133–191
Stickland LH (1951) The determination of small quantities of bacteria by means of the biuret reaction. J Gen Microbiol 5:698–703
Venkateswaran A, McFarlan SC, Ghosal D, Minton KW, Vasilenko A, Makarova K, Wackett LP, Daly MJ (2000) Physiologic determinants of radiation resistance in Deinococcus radiodurans. Appl Environ Microbiol 66:2620–2626
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This work was financially supported by the program DEINOL ISI of OSEO, the French agency for innovation.
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The authors declare that they have no conflict of interest.
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Jean-Louis Uribelarrea and Nathalie Gorret contributed equally to the supervision of this work.
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Bornot, J., Molina-Jouve, C., Uribelarrea, JL. et al. Growth of the extremophilic Deinococcus geothermalis DSM 11302 using co-substrate fed-batch culture. Appl Microbiol Biotechnol 98, 1281–1290 (2014). https://doi.org/10.1007/s00253-013-5397-5
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DOI: https://doi.org/10.1007/s00253-013-5397-5