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Improvements in ethanol production from xylose by mating recombinant xylose-fermenting Saccharomyces cerevisiae strains

  • Applied genetics and molecular biotechnology
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Abstract

To improve the ability of recombinant Saccharomyces cerevisiae strains to utilize the hemicellulose components of lignocellulosic feedstocks, the efficiency of xylose conversion to ethanol needs to be increased. In the present study, xylose-fermenting, haploid, yeast cells of the opposite mating type were hybridized to produce a diploid strain harboring two sets of xylose-assimilating genes encoding xylose reductase, xylitol dehydrogenase, and xylulokinase. The hybrid strain MN8140XX showed a 1.3- and 1.9-fold improvement in ethanol production compared to its parent strains MT8-1X405 and NBRC1440X, respectively. The rate of xylose consumption and ethanol production was also improved by the hybridization. This study revealed that the resulting improvements in fermentation ability arose due to chromosome doubling as well as the increase in the copy number of xylose assimilation genes. Moreover, compared to the parent strain, the MN8140XX strain exhibited higher ethanol production under elevated temperatures (38 °C) and acidic conditions (pH 3.8). Thus, the simple hybridization technique facilitated an increase in the xylose fermentation activity.

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Acknowledgements

The authors would like to thank Ms. Yoshimi Hori for technical assistance. This work has been supported by the Project P07015 administered by the New Energy and Industrial Technology Development Organization (NEDO) under the sponsorship of the Ministry of Economy, Trade, and Industry (METI) of Japan. This work was also supported by Grants-in-Aid for Young Scientists (B) to TH from the Ministry of Education, Culture, Sports, and Technology (MEXT) of Japan and a Special Coordination Funds for Promoting Science and Technology, Creation of Innovative Centers for Advanced Interdisciplinary Research Areas (Innovative Bioproduction Kobe), MEXT, Japan.

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Authors and Affiliations

  1. Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada, Kobe, 657-8501, Japan

    Hiroko Kato, Hiroaki Suyama & Akihiko Kondo

  2. Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada, Kobe, 657-8501, Japan

    Ryosuke Yamada & Tomohisa Hasunuma

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  1. Hiroko Kato
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  2. Hiroaki Suyama
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  3. Ryosuke Yamada
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  4. Tomohisa Hasunuma
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  5. Akihiko Kondo
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Correspondence to Akihiko Kondo.

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Kato, H., Suyama, H., Yamada, R. et al. Improvements in ethanol production from xylose by mating recombinant xylose-fermenting Saccharomyces cerevisiae strains. Appl Microbiol Biotechnol 94, 1585–1592 (2012). https://doi.org/10.1007/s00253-012-3914-6

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  • DOI: https://doi.org/10.1007/s00253-012-3914-6

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