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Bacterial community analysis of swine manure treated with autothermal thermophilic aerobic digestion

  • Environmental Biotechnology
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Abstract

Due to the enviornmental problems associated with disposal of livestock sludge, many stabilization studies emphasizing on the sludge volume reduction were performed. However, little is known about the microbial risk present in sludge and its stabilized products. This study microbiologically explored the effects of anaerobic lagoon fermentation (ALF) and autothermal thermophilic aerobic digestion (ATAD) on pathogen-related risk of raw swine manure by using culture-independent 16S rDNA cloning and sequencing methods. In raw swine manure, clones closely related to pathogens such as Dialister pneumosintes, Erysipelothrix rhusiopathiae, Succinivibrioan dextrinosolvens, and Schineria sp. were detected. Meanwhile, in the mesophilic ALF-treated swine manure, bacterial community clones closely related to pathogens such as Schineria sp. and Succinivibrio dextrinosolvens were still detected. Interestingly, the ATAD treatment resulted in no detection of clones closely related to pathogens in the stabilized thermophilic bacterial community, with the predominance of novel Clostridia class populations. These findings support the superiority of ATAD in selectively reducing potential human and animal pathogens compared to ALF, which is a typical manure stabilization method used in livestock farms.

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Acknowledgment

This research was supported by WCU (World Class University) program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (R33-10076).

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Authors and Affiliations

  1. School of Civil and Environmental Engineering, Yonsei University, Shinchondong 134, Seodaemungu, Seoul, South Korea

    Il Han, Shankar Congeevaram, Dong-Won Ki & Joonhong Park

  2. Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk, South Korea

    Byoung-Taek Oh

  3. WCU Center for Green Metagenomics, Yonsei University, Shinchondong 134, Seodaemungu, Seoul, South Korea

    Shankar Congeevaram & Joonhong Park

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  1. Il Han
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  2. Shankar Congeevaram
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  5. Joonhong Park
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Correspondence to Joonhong Park.

Electronic supplementary material

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Supplementary Table 1

Average removals of organics, volatile suspended solids (VSS), and nutrients from swine manure after 4 days of the pilot-scale ATAD operation (unit = mg/L) (DOC 32 kb)

Supplementary Table 2

Clone sequence IDs and their GenBank accession numbers (DOC 151 kb)

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Han, I., Congeevaram, S., Ki, DW. et al. Bacterial community analysis of swine manure treated with autothermal thermophilic aerobic digestion. Appl Microbiol Biotechnol 89, 835–842 (2011). https://doi.org/10.1007/s00253-010-2893-8

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  • DOI: https://doi.org/10.1007/s00253-010-2893-8

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