Abstract
The present work reports the integration of polymer matrix-supported nanomaterial and enzyme biotechnology for development of industrially feasible biocatalysts. Aqueous leaf extract of Mesua ferrea L. was used to prepare silver nanoparticles distributed within a narrow size range (1–12 nm). In situ oxidative technique was used to obtain poly(ethylene glycol)-supported iron oxide nanoparticles (3–5 nm). Sonication-mediated mixing of above nanoparticles generated the immobilization system comprising of polymer-supported silver–iron oxide nanoparticles (20–30 nm). A commercially important enzyme, Aspergillus niger amyloglucosidase was coupled onto the immobilization system through sonication. The immobilization enzyme registered a multi-fold increment in the specific activity (807 U/mg) over the free counterpart (69 U/mg). Considerable initial activity of the immobilized enzyme was retained even after storing the system at room temperature as well as post-repeated magnetic recycling. Evaluation of the commendable starch saccharification rate, washing performance synergy with a panel of commercial detergents, and antibacterial potency strongly forwards the immobilized enzyme as a multi-functional industrially feasible system.
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Acknowledgements
Mr. Rocktotpal Konwarh sincerely acknowledges the receipt of his Junior Research Fellowship from the Department of Biotechnology, New Delhi. RSIC, NEHU, Shillong and CIF, and IIT Guwahati are thankfully acknowledged for the TEM imaging and magnetometric studies respectively.
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Konwarh, R., Kalita, D., Mahanta, C. et al. Magnetically recyclable, antimicrobial, and catalytically enhanced polymer-assisted “green” nanosystem-immobilized Aspergillus niger amyloglucosidase. Appl Microbiol Biotechnol 87, 1983–1992 (2010). https://doi.org/10.1007/s00253-010-2658-4
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DOI: https://doi.org/10.1007/s00253-010-2658-4