Skip to main content

Advertisement

Log in

A novel approach to microbial breeding—low-energy ion implantation

  • Mini-Review
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

Low-energy ions exist widely in the natural world. People had neglected the interaction between low-energy ions and material; it was even more out of the question to study the relation of low-energy ions and the complicated organism until the biological effects of low-energy ion implantation were discovered in 1989. Nowadays, the value of low-energy ion beam implantation, as a new breeding way, has drawn extensive attention of biologists and breeding experts. In this review, the understanding and utilization of microbial breeding by low-energy ion beam irradiation is summarized, including the characteristics of an ion beam bioengineering facility, present status of the technology of low-energy ions for microbial breeding, and new insights into microbial biotechnology.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Anuntalabhochai S, Chandej R, Phanchaisri B, Yu LD, Vilaithong T, Brown IG (2001) Ion-beam-induced deoxyribose nucleic acid transfer. Appl Phys Lett 78:2393–2395

    Article  CAS  Google Scholar 

  • Cao XH, Du BB, Lu MF (2005) Study on the effect of Aspergillus oryzae mutagenized by N implantation. Journal of Chinese Institute of Food Science and Technology 5:128–132 (in Chinese)

    CAS  Google Scholar 

  • Chen Y, Lin ZX, Zou ZY, Zhang F, Liu D, Liu XH, Tang JZ, Zhu WM, Huang B (1998) High yield antibiotic producing mutants of Streptomyces erythreus induced by low energy ion implantation. Nucl Instrum Methods Phys Res, B Beam Interact Mater Atoms 140:341–348

    Article  CAS  Google Scholar 

  • Chen ZH, Ye Q, Yin GL (2000) Mutation of Candida tropicalis implanted by nitrogen ion and its effect in long chain dicarboxylic acid yield. Microbiology 27:174–177 (in Chinese)

    Google Scholar 

  • Chen XL, Tang XY, Zhou LY (2005) The breeding of the SOD high-producing strain from Bacillus subtilis and studies on culture conditions for SOD production. Acta Laser Biology Sinica 14:173–176 (in Chinese)

    CAS  Google Scholar 

  • Cheng BJ, Kan XZ, Zhu SW, Li PJ (2001) A preliminary study on DNA mutation induction of maize pollen implanted by low energy N beam. Plasma Sci Technol 3:659–664

    Article  CAS  Google Scholar 

  • Feng HY, Wu LJ, Xu A, Hu BR, Hei TK, Yu ZL (2004) Survival of mammalian cells under high vacuum condition for ion bombardment. Cryobiology 49:241–249

    Article  CAS  PubMed  Google Scholar 

  • Ge CM, Gu SB, Zhou XH, Yao JM, Pan RR, Yu ZL (2004) Breeding of l-()-lactic acid producing strain by low-energy ion implantation. J Microbiol Biotechnol 14:363–366

    CAS  Google Scholar 

  • Gu SB, Yao JM, Yuan QP, Xue PJ, Zheng ZM, Wang L, Yu ZL (2006) A novel approach for improving the productivity of ubiquinone-10 producing strain by low-energetic ion beam irradiation. Appl Microbiol Biotechnol 72:456–461

    Article  CAS  PubMed  Google Scholar 

  • Hase Y, Tanaka A, Narumi I, Watanabe H, Inoue M (1998) Development of pollen-mediated gene transfer technique using penetration controlled irradiation with ion beams. JAERI Rev 98–116:81–83

    Google Scholar 

  • Hui YQ, Huang JX, Kong SX (2001) Selective breeding of microbial strain with high yield of α-acetolactate decarboxylase by ion implantation. Journal of Northwest University (Natural Science Edition) 31:251–254 (in Chinese)

    CAS  Google Scholar 

  • Jiang HB, Wang J, Yao JM, Yu ZL (2000) A study on producing ergosterol yeast induced by ions implantation. Dev Ind Microbiol 30:1–3 (in Chinese)

    CAS  Google Scholar 

  • Li DQ, Nie FY, Wei LH, Wei BQ, Chen ZY (2007) Screening of high-yielding biocontrol bacterium Bs-916 mutant by ion implantation. Appl Microbiol Biotechnol 75(6):1401–1408

    Article  CAS  PubMed  Google Scholar 

  • Li SC, Wu M, Yao JM, Pan RR, Yu ZL (2005) Mutation-screening in xylanase-producing strains by ion implantation. Plasma Sci Technol 7:2697–2700

    Article  CAS  Google Scholar 

  • Lin WC, Yin GL, Qiao CH, Ye Q (2001) Production of vitamin C precusor 2-keto-l-gulonic acid from d-sorbitol by mixed culture of microorganisms. Acta Microbiologica Sinica 41:709–715 (in Chinese)

    PubMed  Google Scholar 

  • Liu J, Yao JM (2004) Study on mutagenic breeding of Bacillus subtilis and properties of its antifungal substances. Plasma Sci Technol 6:2433–2436

    Article  CAS  Google Scholar 

  • Liu J, Liu M, Wang J, Yao JM, Pan RR, Yu ZL (2005) Enhancement of the Gibberella zeae growth inhibitory lipopeptides from a Bacillus subtilis mutant by ion beam implantation. Appl Microbiol Biotechnol 69:223–228

    Article  CAS  PubMed  Google Scholar 

  • Mao PH, Lou K, Jin X (2007) Total DNA of Chinese ephedra transferred to yeast by ion beam. Chinese patent No.200610011402.

  • Morishita T, Yamaguchi H, Degi K, Shikazono N, Hase Y, Tanka A, Abe T (2003) Dose response and mutation induced by ion beam irradiation in buckwheat. Nucl Instrum Methods Phys Res, B Beam Interact Mater Atoms 206:565–569

    Article  CAS  Google Scholar 

  • Nie F, Liu CY, Chen XJ (1991) Preliminary study of damage effects of nitrogen ion beam on tomato seed. Journal of Anhui Agricultural Sciences 49:280–281

    Google Scholar 

  • Okamura M, Yasuno N, Ohtsuka M, Tanaka A, Shikazono N, Hase Y (2003) Wide variety of flower-color and -shape mutants regenerated from leaf cultures irradiated with ion beams. Nucl Instrum Methods Phys Res, B Beam Interact Mater Atoms 206:574–578

    Article  CAS  Google Scholar 

  • Phanchaisri B, Yu LD, Anuntalabhochai S, Chandej R, Apavatjrut P, Vilaithong T, Brown IG (2002) Characteristics of heavy ion beam-bombarded bacteria E. coli and induced direct DNA transfer. Surf Coat Technol. 158–159:624–629

    Article  Google Scholar 

  • Sang JL, Zhu LH, Li XH, Shi YF (2002) Screening for excellent mutants of Streptomyces roseoflavus subsp. by N implantation. Bulletin of Science and Technology 18:63–66 (in Chinese)

    Google Scholar 

  • Sangyuenyongpipat S, Yu LD, Tondee N, Wattanavatee T, Vilaithong T, Thongleurm C, Phanchaisri B, Brown IG (2002) Biological ion implantation facility. Presented to the 29th IEEE International Conference on Plasma Science, Banff, Alberta, Canada

  • Shen T, Chen HZ, Peng YY, Liu Y, Liu JM (2002) Study of the selection and mutant on glutamic acid D110 strain by ion implantation. China Condiment 9:24–25 (in Chinese)

    Google Scholar 

  • Song DJ, Yu X (1999) Improving-DNA damage repair ability of Escherichia coli to UV-radiosensitivity by DNA-mediated gene-transfer of low-energy. Chin Sci Bull 44:1778–1783

    Article  CAS  Google Scholar 

  • Sun JR, Zhang J, Mao ZW (2002) Improvement of itaconic acid producing strain by ions implantation. Dev Ind Microbiol 32:33–35 (in Chinese)

    CAS  Google Scholar 

  • Tian CX, Meng Y, Jia XM, Li H, Zhu SM (2005) Selection of high oxytetracyline producing strain by nitrogen ion beam injection technique. Chinese Journal of Antibiotics 30:505–506 (in Chinese)

    CAS  Google Scholar 

  • Tian QY, Chen BJ, Dong HP, Chen TH, Yu ZL, He JJ (1992) Effect of ion implantation on the variation of cotton peroxidase isozyme. Journal of Anhui Agriculture College 19:178–181 (in Chinese)

    Google Scholar 

  • Vilaithonga T, Yu LD, Apavatjrutb P, Phanchaisric B, Sangyuenyongpipata S, Anuntalabhochaid S, Browne IG (2004) Heavy ion induced DNA transfer in biological cells. Radiat Phys Chem 71:927–935

    Article  CAS  Google Scholar 

  • Wang J, Jiang HB, Yao JM, Xue XL, Xie HJ, Liu ZL (1998) Mutation breeding of ergosterol-producing yeast strain by ion injection and its fermentation technology. J Microbiol 18:25–28 (in Chinese)

    Google Scholar 

  • Wang FZ, Wu J, Li ZW, Wang YP, Dai GF, Liang QX (2001) Selective breeding for higher yield strains of rifamycin producer Nocardia mediterranei. Journal of Zhengzhou University 33:50–52 (in Chinese)

    Google Scholar 

  • Wang YP, Wang FZ, Li ZW, Wu J, Chen LH, Qin GY, Huo YP (2003) Study on the screening and fermentation of cyclodextrin glucanotransferase high-producing strains. Journal of Zhengzhou Institute of Technology 24:71–73 (in Chinese)

    Google Scholar 

  • Wang SL, Yang LJ, Chen YC, Chi MJ, Jia XC (2004) Selection high-yield alkaline chitinase producing strain by ion-implantation. Journal of Zhengzhou Institute of High Industry (Natural Science) 19:19–21 (in Chinese)

    CAS  Google Scholar 

  • Wang Q, Wang SZ, Kan ZL, Fu J, Zhang W (2005) Mutation breeding of penicillin producing strains by ion implantation. Hebei Medical Journal 27:709 (in Chinese)

    Google Scholar 

  • Wu LF, Yu ZL (2001) Radiobiological effects of a low-energy ion beam on wheat. Radiat Environ Biophys 40:53–57

    Article  CAS  PubMed  Google Scholar 

  • Wu YJ, Wang XD, Liu GF, Xu X, Yu ZL, Deng JG, He JJ (1990) The application of ions implantation on breeding of wide-compatibility rice line. Journal of Nuclear Agricultural Science 11:154–156 (in Chinese)

    Google Scholar 

  • Wu X, Xu WP, Wang L (2002) Study on the mutation breeding of aureonucleomycin for producing strains with the ion implantation. Shanghai Chemical Industry 21:19–21 (in Chinese)

    CAS  Google Scholar 

  • Wu M, Li SC, Yao JM, Pan RR, Yu ZL (2005) Mutant of a xylanase-producing strain of Aspergillus niger in solid state fermentation by low energy ion implantation. World J Microb Biot 21:1045–1049

    Article  CAS  Google Scholar 

  • Xiang D, Li J, Yao JM, Yu ZL (2002) The breeding of Streptomyces spectabilis 1043 by ion beam implantation. Acta Laser Biology Sinica 11:276–279 (in Chinese)

    Google Scholar 

  • Xu A, Yao JM, Yu ZL (1998) Improvement of mixed strains in the fermentation of 2-keto-L-gulonic acid precursor of Vc by ion implantation (I) screening of strain IPPM-1028 for high-yielding 2-keto-gulonic acid. Dev Ind Microbiol 28:21–24 (in Chinese)

    CAS  Google Scholar 

  • Yamaguchi H, Nagatomi S, Morishita T, Degi K, Tanaka A, Shikazono N, Hase Y (2003) Mutation induced with ion beam irradiation in rose. Nucl Instrum Methods Phys Res, B Beam Interact Mater Atoms 206:561–564

    Article  CAS  Google Scholar 

  • Yang H, Liang HQ, Liao W, Zhou HZ (2003) Breeding of over-producing itaconic acid strains in medium with high concentration of initial sugar by ion implantation. Dev Ind Microbiol 33:30–32 (in Chinese)

    CAS  Google Scholar 

  • Yang LF, Yu L, Zhang L (2005) Study on the screening on the transaminase producing bacteria with the ion implantation. Chemistry and Bioengineering 1:16–18 (in Chinese)

    Google Scholar 

  • Yao JM, Wang J, Wang XQ, Yuan CY, Wang WS, Yu ZL (2000) Breeding of arachidonic acid producing strain by ion implantation. Chinese Journal of Biotechnology 16:478–481 (in Chinese)

    CAS  PubMed  Google Scholar 

  • Yi FM, Wu XH, Feng SY, Chen WM (2002) Ion implantation mutation breeding of xanthine oxidase producing strain and its two-step fermentation technology. Acta Laser Biology Sinica 11:137–141 (in Chinese)

    CAS  Google Scholar 

  • Yu ZL (1998) Introduction of ion beam biotechnology (in Chinese), Anhui Scientific Press

  • Yu L, Yu ZL (2002) The application of the new technology in improving of 2-keto-l-gulonic acid strains. High Technology Letter 11:41–46 (in Chinese)

    Google Scholar 

  • Yu L, Yu ZL (2004) Studies on low energy ion beam mediated DNA transformation of 2-keto-l-gulonic acid strains. Nuclear Techniques 27:264–268 (in Chinese)

    CAS  Google Scholar 

  • Yu ZL, He JJ, Deng JJ, Wang XD, Wu YJ, Liu GF (1989) Preliminary studies on the mutagenic mechanism of the ion implantation on rice. Journal of Anhui Agricultural Sciences 39:12–16 (in Chinese)

    Google Scholar 

  • Yu ZL, Deng JG, He JJ (1991) Mutation breeding by ion implantation. Nucl Instrum Methods Phys Res, B Beam Interact Mater Atoms 59/60:705–708

    Article  Google Scholar 

  • Yu ZL, Yang JB, Wu YJ, Cheng B, He JJ, Huo YP (1993) Transferring gus gene into intact rice cells by low energy ion beam. Nucl Instrum Methods Phys Res, B Beam Interact Mater Atoms 80:1328–1331

    Google Scholar 

  • Yu Y, Yao JM, Yu L, Yu ZL, Li K (2002) Studies on the breeding of pigment-producing strain Monascus with N ion implantation. Journal of the Graduate school of Chinese Academy of Science 19:443–446 (in Chinese)

    Google Scholar 

  • Yu L, Zhou J, Yu ZL (2005) Screening of l-lactic acid producing bacteria by low energy ions implantation and preliminary research of fermentation conditions. Acta Laser Biology Sinica 14:184–188 (in Chinese)

    CAS  Google Scholar 

  • Yuan CL, Yao JM, Wang J, Yu ZL, Gong GH, Shang Y, Qian Y (2003) Breeding of arachidonic acid producing strain by low-energy ion implantation and production in large-scale. High Technology Letter 6:22–25 (in Chinese)

    Google Scholar 

  • Zhang X, Wang SL, Chen CT, Wei J, Chen SQ (2002) Mutation breeding of carotenoid-producing Rhodotorula by ions implantation. Journal of Wuxi University of Light Industry 21:347–349 (in Chinese)

    CAS  Google Scholar 

  • Zhang YQ, Lu ZX, Zou XK (2005) 15 keV N implantation to induce mutation of protopectinase-producing Aspergillus sp. Journal of Radiation Research and Radiation Processing 23:140–144 (in Chinese)

    CAS  Google Scholar 

  • Zhao HY, Li Y, Pei HJ, Zhang TC (2001) Study on effect of gentemicin-producers mutagenized and breeded by N implanting. Journal of Tianjin Institute of Technology 17:14–17 (in Chinese)

    Google Scholar 

  • Zhao X, Zhao LQ, Liu CH, Xie H (2004) Selective breeding of microbial strain with high yield of l-lactic acid by ion implantation. Biotechnology 14:24–26 (in Chinese)

    Google Scholar 

  • Zhen XX, Maol K, Wang YF, Liu HJ (1998) Primary study on biological effect of sugar beet seed induced by ion implantation. Seed 99:29–30 (in Chinese)

    Google Scholar 

  • Zhen WJ, Zhang SF, Zeng XX (2002) Research development of high yielding glutamic acid bacterium screening and breeding. Journal of Xinjiang University (Natural Science Edition) 19:209–211 (in Chinese)

    Google Scholar 

  • Zheng LM, Yu JH, Zhu YH, Wang L, Zhan XB (2003) Effects of the N implantation on the strain of polysialic acid. Jiangsu Food & Fermentation 114:3–7 (in Chinese)

    Google Scholar 

  • Zhou XH, Ge CM, Yao JM, Pan RR, Yu ZL (2005) Optimization of l-lactic acid production of Rhizopus oryzae mutant RLC41-6 by ion beam implantation at low-energy. Plasma Sci Technol 7:3078–3080

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Shao-bin Gu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gu, Sb., Li, Sc., Feng, Hy. et al. A novel approach to microbial breeding—low-energy ion implantation. Appl Microbiol Biotechnol 78, 201–209 (2008). https://doi.org/10.1007/s00253-007-1312-2

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00253-007-1312-2

Keywords

Navigation