Abstract
We have identified and characterized in the supernatant of the transition phase of Bacillus thuringiensis var. kurstaki the peptide SKPDT. This peptide was previously identified by in silico analysis by Pottathil and Lazazzera (Front Biosci 8:32–45 2003) as a putative signaling peptide (NprRB) of the Phr family in B. thuringiensis. The chemically synthesized NprRB did not affect the growth kinetics of B. thuringiensis var. kurstaki but stimulated the sporulation, spore release, and transcription of cry1Aa when added to cultures during the transition phase. In fact, when the peptide (100 nM) was added to a culture in transition phase, the transcription of cry1Aa was stimulated almost threefold, mainly from the late promoter BtII, which requires the late-stage sporulation-specific transcription factor σ K. On the other hand, NprRB did not have any effect on B. subtilis. Thus, SKPDT seems to be a signaling peptide specific for B. thuringiensis.
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Acknowledgements
This work was supported by grant no. 6385 from CIAD A.C. to M. de la Torre. A. Aceves-Diez was supported by a CONACYT fellowship. The authors acknowledge the suggestions of M. Islas, E. Valenzuela, and A. Calderon.
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Aceves-Diez, A.E., Robles-Burgueño, R. & de la Torre, M. SKPDT is a signaling peptide that stimulates sporulation and cry1Aa expression in Bacillus thuringiensis but not in Bacillus subtilis . Appl Microbiol Biotechnol 76, 203–209 (2007). https://doi.org/10.1007/s00253-007-0982-0
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DOI: https://doi.org/10.1007/s00253-007-0982-0