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Red Mangrove Propagule Bacterial Communities Vary With Geographic, But Not Genetic Distance

  • Plant Microbe Interactions
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Abstract

Bacterial communities associated with plant propagules remain understudied, despite the opportunities that propagules represent as dispersal vectors for bacteria to new sites. These communities may be the product of a combination of environmental influence and inheritance from parent to offspring. The relative role of these mechanisms could have significant implications for our understanding of plant–microbe interactions. We studied the correlates of microbiome community similarities across an invasion front of red mangroves (Rhizophora mangle L.) in Florida, where the species is expanding northward. We collected georeferenced propagule samples from 110 individuals of red mangroves across 11 populations in Florida and used 16S rRNA gene (iTag) sequencing to describe their bacterial communities. We found no core community of bacterial amplicon sequence variants (ASVs) across the Florida range of red mangroves, though there were some ASVs shared among individuals within most populations. Populations differed significantly as measured by Bray–Curtis dissimilarity, but not Unifrac distance. We generated data from 6 microsatellite loci from 60 individuals across 9 of the 11 populations. Geographic distance was correlated with beta diversity, but genetic distance was not. We conclude that red mangrove propagule bacterial communities are likely influenced more by local environmental acquisition than by inheritance.

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Data Availability

The datasets generated during and/or analyzed during the current study will be available in the NCBI SRA Database repository under submission numbers PRJNA844062 and PRJNA842720 upon acceptance of publication. [https://www.ncbi.nlm.nih.gov/sra/PRJNA844062, https://www.ncbi.nlm.nih.gov/sra/PRJNA842720].

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Acknowledgements

The authors thank the following individuals and organizations: Katelyn Desorcy-Scherer, Olivia Mason, Scott Steppan, Emily Lemmon, Sophie McCoy, Peter Beerli, Fritz Pichardo, Natasza Fontaine, Prashant Singh, Amber Brown, Steven Miller, Catalina Cuellar-Gempeler, Jackson Powell, Kevin Olson, Natali Ramirez-Bullon, Cheston Peterson, Brian Moe, Catelyn Snyder, the Apalachicola National Estuarine Reserve, the Florida Department of Environmental Protection, the FSU Coastal and Marine Lab, the FSU Department of Biological Sciences, the FSU Graduate School, FNPS, and FNPS Magnolia Chapter.

Funding

This work was supported by the Florida Native Plant Society (FNPS); FNPS Magnolia Chapter; Florida State University (FSU) Department of Biological Sciences; and the FSU Graduate School.

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  1. Department of Biological Science, Florida State University, King Life Sciences Building, 319 Stadium Drive, Tallahassee, Fl, 32304, USA

    Brendan P. Scherer & Austin Mast

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  1. Brendan P. Scherer
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All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Brendan Scherer. The first draft of the manuscript was written by Brendan Scherer, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Brendan P. Scherer.

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Scherer, B.P., Mast, A. Red Mangrove Propagule Bacterial Communities Vary With Geographic, But Not Genetic Distance. Microb Ecol 86, 1010–1022 (2023). https://doi.org/10.1007/s00248-022-02147-w

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