Skip to main content
SpringerLink
Log in

Protection Against Cu(II)-Induced Oxidative Stress and Toxicity to Chlorella vulgaris by 2,2′-Bipyridine-5,5′-dicarboxylic Acid

  • Published:
Archives of Environmental Contamination and Toxicology Aims and scope Submit manuscript

Abstract

In this study, we evaluated the role of 2,2′-bipyridine-5,5′-dicarboxylic acid (Bpy-COOH) in protecting Chlorella vulgaris from the oxidative stress and toxicity induced by Cu(II). Both in vivo and in vitro tests were performed. Different addition orders of Bpy-COOH and Cu(II) were tried in the former, whereas different Bpy-COOH concentrations were used in both experiments. The in vivo experiments showed that the production of reactive oxygen species in C. pyrenoidosa treated by the addition of Bpy-COOH and Cu(II) in three orders were all significantly less than that in cases treated with only Cu(II). In vitro tests indicated that peroxidase-like complexes could be formed between Bpy-COOH and Cu(II). Based on these results, it could be concluded that the use of Bpy-COOH could significantly decrease Cu(II) toxicity to algal cells by forming peroxidase-like complexes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  • Amuda OS, Giwa AA, Bello IA (2007) Removal of heavy metal from industrial wastewater using modified activated coconut shell carbon. Biochem Eng J 36:174–181

    Article  CAS  Google Scholar 

  • Bai DR, Baumgartner T (2010) Synthesis and photophysical properties of bipyridine-extended dithienophosphole chromophores for transition metal complexation. Organometallics 29:3289–3297

    Article  CAS  Google Scholar 

  • Chen H, Chen J, Guo YN, Wen YZ, Liu J, Liu WP (2012) Evaluation of the role of the glutathione redox cycle in Cu(II) toxicity to green algae by a chiral perturbation approach. Aquat Toxicol 15:120–121

    Google Scholar 

  • Chen H, Sheng XL, Wen YZ, Zhang LJ, Bao HL, Lin LN et al (2013) New insights into the effect of the herbicide imazethapyr on Cu(II) ecotoxicity to the aquatic unicellular alga Scenedesmus obliquus. Aquat Toxicol 140–141:407–417

    Article  Google Scholar 

  • Chong KH, Volesky B (1995) Description of two metal biosorption equilibria by Langmuir-type models. Biotechnol Bioeng 47:451–460

    Article  CAS  Google Scholar 

  • Cid A, Herrero C, Torres E, Abalde J (1995) Copper toxicity on the marine microalga Phaeodactylum tricornutum: effects on photosynthesis and related parameters. Aquat Toxicol 31:165–174

    Article  CAS  Google Scholar 

  • Gyulkhandanyan AV, Feeney CJ, Pennefather PS (2003) Modulation of mitochondrial membrane potential and reactive oxygen species production by copper in astrocytes. J Neurochem 87:448–460

    Article  CAS  Google Scholar 

  • Halliwell B, Gutteridge JMC (1999) Free radicals in biology and medicine. Oxford University Press, Oxford

    Google Scholar 

  • Katsumata H, Kaneco S, Inomata K, Itoh K, Funasaka K, Masuyama K et al (2003) Removal of heavy metals in rinsing wastewater from plating factory by adsorption with economical viable materials. J Environ Manag 69:187–191

    Article  Google Scholar 

  • Kiran I, Akar T, Tunali S (2005) Biosorption of Pb(II) and Cu(II) from aqueous solutions by pretreated biomass of Neurosporacrassa. Process Biochem 40:3550–3558

    Article  CAS  Google Scholar 

  • Knauert S, Knauer K (2008) The role of reactive oxygen species in copper toxicity to two freshwater green algae. J Phycol 44:311–319

    Article  CAS  Google Scholar 

  • Manevich Y, Held KD, Biaglow JE (1997) Coumarin-3-carboxylicacid as a detector for hydroxyl radicals generated chemically and by gamma radiation. Radiat Res 148:580–591

    Article  CAS  Google Scholar 

  • Maurya RC, Patel P, Rajput S (2003) Synthesis and characterization of mixed-ligand complexes of Cu(II), Ni(II), Co(II), Zn(II), Sm(III), and U(VI)O2, with a schiff base derived from the sulfa drug sulfamerazine and 2,2′-bipyridine. Synth React Inorg Metal Org Chem 33:801–816

    Article  CAS  Google Scholar 

  • Mohanty JG, Jaffe JS, Schulman ES, Raible DG (1997) A highly sensitive fluorescent microassay of H2O2 release from activated human leukocytes using a dihydroxyphenoxazine derivative. J Immunol Methods 202:133–141

    Article  CAS  Google Scholar 

  • Morrison GMP, Florence TM (1989) Comparison of physicochemical speciation procedures with metal toxicity to Chlorella pyrenoidosa. Copper complexation capacity. Electroamlyski 1:107–112

    Article  CAS  Google Scholar 

  • Nasernejad B, Katsumata H, Kaneco S, Inomata K, Itoh K, Funasaka K et al (2005) Comparison for biosorption modeling of heavy metals (Cr(III), Cu (II), Zn (II)) adsorption from wastewater by carrot residues. Process Biochem 40:1319–1322

    Article  CAS  Google Scholar 

  • Natasha MF, Jennifer LS, Scott JM, Richard PL (2000) pH-dependent toxicity of copper and uranium to a tropical freshwater alga (Chlorella sp.). Aquat Toxicol 48:275–289

    Article  Google Scholar 

  • Oswald WJ, Lee EW, Adan B, Yao KH (1978) New wastewater treatment method yields a harvest of saleable algae. WHO Chronicle 32:348–350

    CAS  Google Scholar 

  • Pavasant P (2006) Biosorption of Cu +2 , Cd +2 , Pb2 +, and Zn +2 using dried marine green macroalga Caulerpa lentillifera. Bioresour Technol 97:2321–2329

    Article  CAS  Google Scholar 

  • Pinto E (2003) Heavy metal-induced oxidative stress in algae. J Phycol 39:1008–1018

    Article  CAS  Google Scholar 

  • Razinger J, Razinger J, Dermastia M, Drinovec L, Drobne D, Zrimec A et al (2007) Antioxidative responses of duckweed (Lemna minor L.) to short-term copper exposure. Environ Sci Pollut Res 14:194–201

    Article  CAS  Google Scholar 

  • Schiewer S, Volesky B (1995) Modeling of the proton-metal ion exchange in biosorption. Environ Sci Technol 29:3049–3058

    Article  CAS  Google Scholar 

  • Schützendübel A, Polle A (2002) Plant responses to abiotic stresses: heavy metal-induced oxidative stress and protection by mycorrhization. J Exp Bot 53:1351–1365

    Article  Google Scholar 

  • Shen CS, Chen H, Wu SS, Wen YZ, Li LN, Jiang Z et al (2013) Highly efficient detoxification of Cr(VI) by chitosan-Fe(III) complex: process and mechanism studies. J Hazard Mater 244–245:689–697

    Article  Google Scholar 

  • Siaut M, Cuiné S, Cagnon C, Fessler B, Nguyen M, Carrier P et al (2001) Oil accumulation in the model green alga Chlamydomonas reinhardtii: characterization, variability between common laboratory strains and relationship with starch reserves. BMC Biotechnol 11:7

    Article  Google Scholar 

  • Sigel H (1969) Catalase and peroxidase activity of Cu +2 complexes. Angew Chem 8:167–177

    Article  CAS  Google Scholar 

  • Sigel H, Flierl C, Griesser R (1968) On the kinetics and mechanism of the decomposition of hydrogen peroxide, catalyzes by the Cu +2 -2,2′-bipyridyl complex. J Am Chem Soc 91:1061–1064

    Article  Google Scholar 

  • Siripornadulsil S, Siripornadulsil S, Traina S, Verma DP, Sayre RT (2002) Molecular mechanisms of proline-mediated tolerance to toxic heavy metals in transgenic microalgae. Plant Cell 14:284–2837

    Article  Google Scholar 

  • Skowroński T (1986) Sorption of cadmium on green microalga Stichococcus bacillaris. Chemosphere 15:69–76

    Article  Google Scholar 

  • Stauber JL, Florence TM (1987) Mechanism of toxicity of ionic copper and copper complexes to algae. Mar Biol 94:511–519

    Article  CAS  Google Scholar 

  • Stohs SJ, Bagchi D (1995) Oxidative mechanisms in the toxicity of metal ions. Free Radic Biol Med 18:321–336

    Article  CAS  Google Scholar 

  • Szivák I, Behra R, Laura Sigg (2009) Metal-induced reactive oxygen species production in Chlamydonas reinhardtii (Chlorophyceae). J Phycol 45:427–435

    Article  Google Scholar 

  • Terry PA, Stone W (2002) Biosorption of cadmium and copper contaminated water by Scenedesmus abundans. Chemosphere 47:249–255

    Article  CAS  Google Scholar 

  • Tsuji N, Hirayanagi N, Okada M, Miyasaka H, Hirata K, Zenk MH et al (2002) Enhancement of tolerance to heavy metals and oxidative stress in Dunaliella tertiolecta by Zn-induced phytochelatin synthesis. Biochem Biophys Res 293:653–659

    Article  CAS  Google Scholar 

  • Veglio F, Beolchini F (1997) Removal of metals by biosorption: a review. Hydrometallurgy 44:301–316

    Article  CAS  Google Scholar 

  • Wang L, Min M, Li Y, Chen P, Chen Y, Liu Y et al (2010) Cultivation of green algae Chlorella sp. in different wastewaters from municipal wastewater-treatment plant. Appl Biochem Biotechnol 162:1174–1186

    Article  CAS  Google Scholar 

  • Wen YZ, Yuan YL, Chen H, Xu DM, Lin KD, Liu WP (2010) Effect of chitosan on the enantioselective bioavailability of the herbicide dichlorprop to Chlorella pyrenoidosa. Environ Sci Technol 44:4981–4987

    Article  CAS  Google Scholar 

  • Wen YZ, Chen H, Shen CS, Zhao MR, Liu WP (2011) Enantioselectivity tuning of chiral herbicide dichlorprop by copper: roles of reactive oxygen species. Environ Sci Technol 45:4778–4784

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by the National Natural Science Foundation of China (Grant No. 21377111), the Zhejiang Provincial Natural Science Foundation of China (Grant No. LY12B07006), Shanghai Municipal Natural Science Foundation (Grant No. 13ZR1447800), and the Knowledge Innovation Program of Chinese Academy of Sciences.

Author information

Authors and Affiliations

  1. Institute of Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, Yuhangtang Road #866, Hangzhou, 310058, China

    Yuezhong Wen & Xiaolin Sheng

  2. MOE Key Laboratory of Environmental Remediation & Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, China

    Shufang Song & Weiping Liu

  3. Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201204, China

    Lina Li

Authors
  1. Yuezhong Wen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xiaolin Sheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shufang Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Lina Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Weiping Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yuezhong Wen.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wen, Y., Sheng, X., Song, S. et al. Protection Against Cu(II)-Induced Oxidative Stress and Toxicity to Chlorella vulgaris by 2,2′-Bipyridine-5,5′-dicarboxylic Acid. Arch Environ Contam Toxicol 66, 400–406 (2014). https://doi.org/10.1007/s00244-013-9977-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00244-013-9977-2

Keywords

Search

Navigation