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Effects of Subchronic Coexposure to Arsenic and Endosulfan on the Erythrocytes of Broiler Chickens: A Biochemical Study

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Abstract

Arsenic is a known global groundwater contaminant. The organochlorine insecticide endosulfan has gained significance as an environmental pollutant due to its widespread use in the control of many food- and non-food-crop-damaging insects. The adverse effects produced by arsenic or endosulfan alone in humans and animals are well documented, but very little is known about the consequences of their coexposure. We evaluated whether their simultaneous exposure can induce oxidative stress and affect antioxidative systems and certain membrane-bound enzymes in erythrocytes of broiler chickens. Day-old chicks were exposed to 3.7 ppm of arsenic via drinking water or 30 ppm of endosulfan-mixed feed or similarly coexposed to these in the same dose levels for 60 days. At term, the impact of their coexposure was assessed by evaluating lipid peroxidation (LPO), activities of superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), glutathione-S-transferase (GST), different ATPases and acetylcholinesterase (AChE) in erythrocytes, serum glucose, and levels of glutathione (GSH) and glycosylated hemoglobin (GHb) in blood. LPO was increased with all of the treatments. Catalase was decreased with endosulfan and the coexposure, but not with arsenic, whereas GSH was decreased with arsenic and endosulfan, but not with the coexposure. All of the treatments increased SOD and GPx activities. GST activity was increased only in the coexposed birds. None of the treatments affected the activities of total ATPase and Mg2+-ATPase. Na+-K+-ATPase activity was decreased in the endosulfan-treated and the coexposed birds. All three exposures increased erythrocyte AChE activity. Endosulfan increased the serum glucose level and arsenic and endosulfan increased GHb levels, but these were not altered in the coexposed birds. Erythrocyte protein content was insignificantly decreased with these treatments. Overall, the effects of coexposure were not appreciably different from either of the agents, except on AChE, GSH, and glucose. The results do not reflect any specific type of interaction between these agents in chicken erythrocytes, but they do indicate that the coexposure induces a low level of oxidative stress, which is comparable to that induced by arsenic or endosulfan.

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Acknowledgments

The Junior Research Fellowship awarded to Dr. Manoj Aggarwal by the Indian Council of Agricultural Research (ICAR), New Delhi is gratefully acknowledged. The authors are thankful to M/s Mitsu Crop Sciences Pvt. Ltd., India for the gift of the technical-grade endosulfan and express gratitude to the Director of the Indian Veterinary Research Institute for providing necessary facilities for the present work.

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  1. Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar, UP, 243122, India

    Manoj Aggarwal, Suresh Babu Naraharisetti, S. N. Sarkar, G. S. Rao & J. K. Malik

  2. Institut für Arbeitsphysiologie an der Universität Dortmund, Leibniz Research Centre for Working Environment and Human Factors, Ardeystraβe 67, 44139, Dortmund, Germany

    Manoj Aggarwal & G. H. Degen

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  1. Manoj Aggarwal
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Aggarwal, M., Naraharisetti, S.B., Sarkar, S.N. et al. Effects of Subchronic Coexposure to Arsenic and Endosulfan on the Erythrocytes of Broiler Chickens: A Biochemical Study. Arch Environ Contam Toxicol 56, 139–148 (2009). https://doi.org/10.1007/s00244-008-9171-0

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