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Effect of Cadmium(II), Chromium(VI), and Arsenic(V) on Long-Term Viability- and Growth-Inhibition Assays Using Vibrio fischeri Marine Bacteria

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Abstract

As a complement to previous results obtained using the standard Microtox acute-toxicity test, which is based on measuring the rapid decrease of bioluminescence (5 to 30 minutes of exposure) in Vibrio fischeri bacteria in the presence of toxicants, the long-term effects of Cd(II), Cr(VI), and As(V) were studied on growth rate and viability assays of the same bacteria adapted to longer-lasting cultures, i.e., 48 or 72 hours instead of 5 or 30 minutes. Effects on viability or growth, as studied by establishing dose- and time-response curves, confirmed that these poisonous chemicals were not particularly toxic to these bacteria. Nevertheless, in the case of Cr(VI), the viability-inhibition assay appeared to be more sensitive than the Microtox acute-toxicity test. Interestingly, it was possible to observe a clear hormesis phenomenon, especially for Cd(II), under the conditions of both viability- and growth-inhibition assays.

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Acknowledgments

Thanks are due to Wouter Marcelis for helping in the experimental work. This research was supported by Ministerio de Ciencia y Tecnología, Spain (Project No. PPQ2002-04131-C02-02). The authors also thank Generalitat de Catalunya, Spain, for the grant for researchers mobility (Grant No. ACI2003-52).

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Authors and Affiliations

  1. Department of Chemical Engineering, University of Girona, Avda. Lluís Santaló, s/n, Girona, 17071, Spain

    E Fulladosa & I. Villaescusa

  2. Laboratory of Cell Biology Faculty of Medicine UPS, J. C. Murat, 37 Allées Jules Guesde, Toulouse, 31073, France

    J. C. Murat

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  1. E Fulladosa
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  2. J. C. Murat
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  3. I. Villaescusa
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Correspondence to I. Villaescusa.

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Fulladosa, E., Murat, J.C. & Villaescusa, I. Effect of Cadmium(II), Chromium(VI), and Arsenic(V) on Long-Term Viability- and Growth-Inhibition Assays Using Vibrio fischeri Marine Bacteria. Arch Environ Contam Toxicol 49, 299–306 (2005). https://doi.org/10.1007/s00244-004-0170-5

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  • DOI: https://doi.org/10.1007/s00244-004-0170-5

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