Skip to main content
SpringerLink
Log in

Effect of lisinopril on renal tissue damage in unilateral ureteral obstruction in rats

  • Original Paper
  • Published:
Urological Research Aims and scope Submit manuscript

Abstract

In this study, it was aimed to investigate apoptosis in renal injury and the effect of lisinopril in rat model, which constitute unilateral ureteral obstruction. The retroperitoneal ureter was ligated with a 4.0 silk for the experimental model of ureteral obstruction in Wistar albino rats. Untreated group (n = 20) received no treatment. For the lisinopril-treated group (n = 20), 20 mg/kg/day of drug was given orally. Ultrastructural differences were analyzed using electron microscopic technique; apoptotic distribution was analyzed using the TUNEL method. After electron microscopic evaluation, on the 4th and 14th day in the untreated group, edema in the glomeruli, loss of microvillus and apoptotic cells in proximal tubule cells and sclerosis in the glomeruli were detected. On the 4th day in the lisinopril-treated group, the kidney was ultrastructurally normal and a less number of apoptotic cells were only observed on the 14th day. On light microscopic examination on the 4th and 14th day in the untreated group, while the glomeruli were normal in structure, the boundary of the proximal tubule was disrupted and some picnotic cells in both the proximal and collecting tubules were observed. In both 4th and 14th day of the lisinopril-treated group, kidney showed normal structure, although in some places picnotic cells in the collecting tubules were observed. In conclusion, lisinopril was effective and it may prevent early renal damage in the direct obstruction model.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Chavalier Robert L (2006) Pathogenesis of renal injury in obstructive uropathy. Curr Opin Pediatr 18:153–160

    Article  Google Scholar 

  2. Klahr S, Buekert J, Purkerson ML (1977) The kidney in obstructive uropathy. Contrib Nephrol 7:220

    PubMed  CAS  Google Scholar 

  3. Jones EA, Shahed A, Shoskes DA (2000) Modulation of apoptotic and inflammatory genes by bioflavonoids and angiotensin II inhibition in ureteral obstruction. Urology 56:346

    Article  PubMed  CAS  Google Scholar 

  4. Turan T, Van Harten R, De Water R, Tuncay OL, Kok DJ (2003) Is enalapril adequate for the prevention of renal tissue damage caused by unilateral ureteral obstruction and/or hyperoxaluria? Urol Res 31:212–217

    Article  PubMed  CAS  Google Scholar 

  5. Fronkier J (2005) Pharmacologic intervention in urinary tract obstruction––is it possible? Kidney Int 68:894–895

    Article  Google Scholar 

  6. El Chaar M, Chen J, Seshan VS, Jha S, Richardson I, Ledbetter SR, Vaughan ED, Poppas DP, Felsen D (2007) Effect of combination therapy with enalapril and the TGF-β antagonist 1D11 in unilateral ureteral obstruction. Am J Physiol Renal Physiol 292:F1291–F1301

    Article  PubMed  CAS  Google Scholar 

  7. Klahr S (2001) Urinary tract obstruction. Semin Nephrol 21:133–145

    Article  PubMed  CAS  Google Scholar 

  8. Radovic N, Cuzic S, Knotek M (2008) Effect of unilateral ureteral obstruction and anti-angiotensin II treatment on renal tubule and interstitial cell apoptosis in rats. Croat Med J 49:600–607

    Article  PubMed  CAS  Google Scholar 

  9. Cummings MC, Winterford CM, Walker NI (1997) Apoptosis. In: Stenberg SS (ed) Histology for pathologists, 2nd edn. Lippincott-Raven Publishers, Philadelphia, pp 3–21

    Google Scholar 

  10. Bowen ID (1993) Apoptosis or programmed cell death. Cell Biol Int 17:365–380

    Article  PubMed  CAS  Google Scholar 

  11. Stellar H (1995) Mechanisms and genes of cellular suicide. Science 267:1445–1449

    Article  Google Scholar 

  12. Nagata S, Golstein P (1995) The Fas death factor. Science 267:1449–1456

    Article  PubMed  CAS  Google Scholar 

  13. Borutaite V (2010) Mitochondria as decision-makers in cell death. Environ Mol Mutagen 51(5):406–416

    PubMed  CAS  Google Scholar 

  14. Baker AJ, Mooney A, Hughes J et al (1994) Mesangial cell apoptosis: the major mechanism for resolution of glomerular hypercellularity in experimental mesangial proliferative nephritis. J Clin Invest 94:2105–2116

    Article  PubMed  CAS  Google Scholar 

  15. Gonçalves GR, Biato AM, Colosimo DGR, Martinusso AC, Pecly ID, Farias KE, Cardoso RL, Takiya MC, Ornellas FRJ, Leite M Jr (2004) Effects of myophenolate moferil and lisinopril on collagen deposition in unilateral ureteral obstruction in rats. Am J Nephrol 24:527–536

    Article  PubMed  Google Scholar 

  16. Beck AD (1971) The effect of intra-uterine urinary obstruction upon the development of the fetal kidney. J Urol 105:784

    PubMed  CAS  Google Scholar 

  17. Berman DJ, Maizels M (1982) The role of urinary obstruction in the genesis of renal dysplasia. A model in the chick embryo. J Urol 128:1091

    PubMed  CAS  Google Scholar 

  18. Mc Vary KT, Maizels M (1989) Urinary obstruction reduces glomerulogenesis in the developing kidney: a model in the rabbit. J Urol 142:646

    CAS  Google Scholar 

  19. Steinhardt GF, Salinas-Madrigal L, Farber R, Phillips B, Vogler G (1994) Experimental ureteral obstruction in the fetal opossum: histologic assessment. J Urol 152:2133

    PubMed  CAS  Google Scholar 

  20. Wen JG, Frokiaer J, Jorgensen TM, Djurhuus JC (1999) Obstructive nephropathy: an update of the experimental research. Urol Res 27:29–39

    Article  PubMed  CAS  Google Scholar 

  21. Ward RM, Starr NT, Snow BW, Bellinger MF, Pysher TJ, Zaino RJ (1989) Serial renal function in an ovine model of unilateral fetal urinary tract obstruction. J Urol 142:652

    PubMed  CAS  Google Scholar 

  22. Fink RL, Caridis DT, Chmiel R, Ryan G (1980) Renal impairment and its reversibility following variable periods of complete ureteric obstruction. Aust NZ J Surg 50:77

    Article  CAS  Google Scholar 

  23. Moody TE, Vaughan ED Jr, Gillenwater JY (1977) Comparison of the renal hemodynamic response to unilateral and bilateral ureteric occlusion. Invest Urol 14:455

    PubMed  CAS  Google Scholar 

  24. Miyajima A, Chen J, Poppas DP, Vaughan ED Jr, Felsen D (2001) Role of nitric oxide in renal tubular apoptosis of unilateral obstruction. Kidney Int 59:1290–1303

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Urology, Nizip Public Hospital, Gaziantep, Turkey

    İlker Karabuga

  2. Department of Urology, Tepecik Training Hospital, Izmir, Turkey

    Kaan Akbay

  3. Department of Urology, Faculty of Medicine, Ege University, Izmir, Turkey

    Burak Turna, Barış Altay, Oktay Nazli & Erdal Apaydin

  4. Department of Histology and Embryology, Faculty of Medicine, Celal Bayar University, Manisa, Turkey

    H. Seda Vatansever & Elgin Turkoz Uluer

  5. Department of Histology and Embryology, Faculty of Medicine of Cerrahpasa, İstanbul University, Istanbul, Turkey

    Elif Güzel

  6. Department of Urology, Turgutlu Ege Umut Hospital, Manisa, Turkey

    Güven Ustun

  7. Department of Urology, Oltu Public Hospital, Erzurum, Turkey

    Fatih Ekren

  8. Department of Histology and Embryology, Faculty of Medicine, Hacettepe University, Ankara, Turkey

    Sevda Muftuoglu

  9. 915 sokak No: 159/7, Bornova, 35030, Izmir, Turkey

    Kaan Akbay

Authors
  1. İlker Karabuga
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kaan Akbay
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Burak Turna
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. H. Seda Vatansever
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Barış Altay
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Elif Güzel
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Elgin Turkoz Uluer
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Güven Ustun
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Fatih Ekren
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Oktay Nazli
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Sevda Muftuoglu
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Erdal Apaydin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kaan Akbay.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Karabuga, İ., Akbay, K., Turna, B. et al. Effect of lisinopril on renal tissue damage in unilateral ureteral obstruction in rats. Urol Res 40, 27–34 (2012). https://doi.org/10.1007/s00240-011-0393-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00240-011-0393-7

Keywords

Search

Navigation