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Selection and Application of DNA Aptamer Against Oncogene Amplified in Breast Cancer 1

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Abstract

Amplified in breast cancer 1 (AIB1), also known as steroid receptor coactivator 3 (SRC-3), is a transcriptional coactivator that interacts with nuclear receptors and other transcription factors to enhance their effects on target gene transcription. AIB1, which acts as a major oncogene, is highly expressed in many human cancers, and has been demonstrated to be a key regulator for tumor initiation, progression, metastasis, invasion, and survival. Recruitment of the transcriptional factor CBP/p300 by CBP/p300-interaction domain (CID) of AIB1 is essential for its transcriptional activation function. In this research, we isolated a DNA aptamer AY-3 that binds to AIB1-CID from a random oligonucleotide library using in vitro screening technology—Systematic Evolution of Ligands by EXponential enrichment (SELEX). The binding affinity of the aptamer to AIB1-CID fusion protein is in the nanomolar range. More importantly, the aptamer was found to disrupt in the interaction between p300 and AIB1. This aptamer has great potential to serve as a therapeutic agent for cancer by inhibiting the coactivation of AIB1.

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Acknowledgments

We thank the National Basic Research Program of China (2013CB933703), National Science Foundation of China (21325522, 21205100, 21275122, 21521004), National Science Fund for Fostering Talents of Basic Science (J1310024), Fundamental Research Funds for the Central Universities (20720150141), and National Instrumentation Program (2011YQ03012412) for their financial support.

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Authors and Affiliations

  1. State Key Laboratory of Physical Chemistry of Solid Surfaces, The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Collaborative Innovation Center of Chemistry for Energy Materials, Key Laboratory for Chemical Biology of Fujian Province, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, People’s Republic of China

    Yuan An, Jie Wu, Zhi Zhu, Mingxuan Gao & Chaoyong James Yang

  2. State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian, 361102, People’s Republic of China

    Bo Yang & Chundong Yu

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Correspondence to Chundong Yu or Chaoyong James Yang.

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An, Y., Wu, J., Yang, B. et al. Selection and Application of DNA Aptamer Against Oncogene Amplified in Breast Cancer 1. J Mol Evol 81, 179–185 (2015). https://doi.org/10.1007/s00239-015-9703-y

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