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Acid-Sensitive Outwardly Rectifying Anion Channels in Human Erythrocytes

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Abstract

Acid-sensitive outwardly rectifying anion channels (ASOR) have been described in several mammalian cell types. The present whole-cell patch-clamp study elucidated whether those channels are expressed in erythrocytes. To this end whole-cell recordings were made in human erythrocytes from healthy donors treated with low pH and high osmotic pressure. When the pipette solution had a reduced Cl− concentration, treatment of the cells with Cl−-containing normal and hyperosmotic (addition of sucrose and polyethelene glycol 1000 [PEG-1000] to the Ringer) media with low pH significantly increased the conductance of the cells at positive voltages. Channel activity was highest in the PEG-1000 media (95 and 300 mM PEG-1000, pH 4.5 and 4.3, respectively) where the current–voltage curves demonstrated strong outward rectification and reversed at −40 mV. Substitution of the Cl−-containing medium with Cl−-free medium resulted in a decrease of the conductance at hyperpolarizing voltages, a shift in reversal potential (to 0 mV) and loss of outward rectification. The chloride currents were inhibited by chloride channels blockers DIDS and NPPB (IC50 for both was ~1 mM) but not with niflumic acid and amiloride. The observations reveal expression of ASOR in erythrocytes.

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Acknowledgements

We thank Dr. Ekaterina Shumilina for helpful advice and Prof. Dr. Ingolf Bernhardt for helpful discussion of the manuscript.

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Authors and Affiliations

  1. Department of Physiology, University of Tübingen, Gmelinstr. 5, 72076, Tübingen, Germany

    Yuliya V. Kucherenko & Florian Lang

  2. Department of Cryobiophysics, Institute for Problems of Cryobiology and Cryomedicine of the Ukrainian National Academy of Sciences, 61015, Kharkov, Ukraine

    Yuliya V. Kucherenko

  3. Laboratory of Biophysics, Faculty of Natural and Technical Sciences III, Saarland University, Saarbruecken, Germany

    Daniel Mörsdorf

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  1. Yuliya V. Kucherenko
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  2. Daniel Mörsdorf
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  3. Florian Lang
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Correspondence to Florian Lang.

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Kucherenko, Y.V., Mörsdorf, D. & Lang, F. Acid-Sensitive Outwardly Rectifying Anion Channels in Human Erythrocytes. J Membrane Biol 230, 1–10 (2009). https://doi.org/10.1007/s00232-009-9179-z

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  • DOI: https://doi.org/10.1007/s00232-009-9179-z

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