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Relationships between RNA content and egg production rate in Acartia bifilosa (Copepoda, Calanoida) of different spatial and temporal origin

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An Erratum to this article was published on 15 January 2008

Abstract

To evaluate factors regulating RNA content (RNA, μg RNA female−1) – egg production rate (EPR, eggs female−1 day−1) relationship and to develop a model for in situ egg production rate estimates for Acartia bifilosa, we (1) measured EPR and RNA in females sampled at geographically distant areas at varying temperature (T, °C), (2) determined environmental (station, season, and T), endogenous (prosome length (PL), mm and EPR) variables that influence RNA levels, and (3) explored a set of multiple regression models to predict EPR from RNA, PL, station, season, and T. Egg production experiments were carried out in spring and summer 2005 in the Gulf of Finland and in the western part of the northern Baltic proper. We found that up to 88% of the RNA variation could be explained by variations in PL, EPR, and season/T. In explaining the RNA variability, PL played a major role followed in order of importance by EPR and season/T. Further, PL, RNA, and season/T explained up to 53% of the variation in EPR, nearly half of which is explained by RNA alone. The effect of spatial origin was never significant, suggesting that the derived relationships are general for A. bifilosa inhabiting northern Baltic proper.

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References

  • Ackefors H (1969) Ecological zooplankton investigations in the Baltic Proper 1963–1965. Carl Bloms boktryckeri AB, Lund, pp 1–139

    Google Scholar 

  • Arrhenius F (1996) Diet composition and food selectivity of 0-group herring (Clupea harengus L.) and sprat (Sprattus sprattus (L.)) in the northern Baltic Sea. ICES J Mar Sci 53:701–712

    Article  Google Scholar 

  • Bergeron J-P (1997) Nucleic acids in ichthyoplankton ecology: a review, with emphasis on recent advances for new perspectives. J Fish Biol 51(suppl.a):284–302

    Article  CAS  Google Scholar 

  • Brown JH, Gillooly JF, Allen AP, Savage VM, West GB (2004) Toward a metabolic theory of ecology. Ecology 85(7):1771–1789

    Article  Google Scholar 

  • Buckley L, Caldarone E, Ong T-L (1999) RNA–DNA ratio and other nucleic acid-based indicators for growth and condition of marine fishes. Hydrobiologia 401:265–277

    Article  CAS  Google Scholar 

  • Caldarone EM, St Onge-Burns JM, Buckley LJ (2003) Relationship of RNA/DNA ratio and temperature to growth in larvae of Atlantic cod Gadus morhua. Mar Ecol Prog Ser 262:229–240

    Article  CAS  Google Scholar 

  • Caldarone EM, Clemmesen CM, Berdalet E, Miller TJ, Folkvord A, Holt GJ, Olivar MP, Suthers IM (2006) Intercalibration of four spectrofluorometric protocols for measuring RNA/DNA ratios in larval and juvenile fish. Limnol Oceanogr Meth 4:153–163

    Article  CAS  Google Scholar 

  • Calliari D, Andersen CM, Thor P, Gorokhova E, Tiselius P (2006) Salinity modulates the energy balance and reproductive success of co-occuring copepods Acartia tonsa and A. Clausi in different ways. Mar Ecol Prog Ser 312:177–188

    Article  Google Scholar 

  • Carlotti F, Nival P (1992) Model of copepod growth and development: moulting and mortality in relation to physiological processes during an individual moult cycle. Mar Ecol Prog Ser 84:219–233

    Article  Google Scholar 

  • Cunha I, Saborido-Rey F, Planas M (2003) Use of multivariate analysis to assess the nutritional condition of fish larvae from nucleic acids and protein content. Biol Bull 204:339–349

    Article  CAS  Google Scholar 

  • Dahl U, Gorokhova E, Breitholtz M (2006) Application of growth-related sublethal endpoints in ecotoxicological assessments using a harpacticoid copepod. Aquat Toxicol 77:433–438

    Article  CAS  Google Scholar 

  • Gonzalez CRM, Bradley BP (1994) Salinity stress proteins in Eurytemora affinis. Hydrobiologia 292/293:461–468

    Article  CAS  Google Scholar 

  • Gorokhova E (1999) Vertical structures in the Baltic pelagic ecosystem. In: Proceedings of the third BASYS annual science conference, SP2-5, paper abstracts: 20

  • Gorokhova E (2003) Relationships between nucleic acid levels and egg production rates in Acartia bifilosa: implications for growth assessment of copepods in situ. Mar Ecol Prog Ser 262:163–172

    Article  CAS  Google Scholar 

  • Gorokhova E (2005) Effects of preservation and storage of microcrustaceans in RNAlater on RNA and DNA degradation. Limnol Ocanogr Meth 3:143–148

    Article  CAS  Google Scholar 

  • Gorokhova E, Kyle M (2002) Analysis of nucleic acids in Daphnia: development of methods and ontogenetic variations in RNA–DNA content. J Plankton Res 24(5):511–522

    Article  CAS  Google Scholar 

  • Hansen FC, Möllmann C, Schütz U, Neumann T (2006) Spatio-temporal distribution and production of calanoid copepods in the central Baltic Sea. J Plankton Res 28(1):39–54

    Article  Google Scholar 

  • Ibiam U, Grant A (2005) RNA/DNA ratios as a sublethal endpoint for large-scale toxicity tests with the nematode Caenorhabditis elegans. Environ Toxicol Chem 24(5):1155–1159

    Article  CAS  Google Scholar 

  • Johansson S, Hanssson S, Orfelina A-N (1993) Temporal and spatial variation of coastal zooplankton in the Baltic Sea. Ecography 16:167–173

    Article  Google Scholar 

  • Kay AD, Ashton IW, Gorokhova E, Kerkhoff AJ, Liess A, Litchman E (2005) Toward a stoichiometric framework for evolutionary biology. Oikos 109:6–17

    Article  Google Scholar 

  • Kono N, Tsukamoto Y, Zenitani H (2003) RNA:DNA ratio for diagnosis of the nutritional condition of Japanese anchovy Engraulis japonicus larvae during the first-feeding stage. Fish Sci 69:1096–1102

    Article  CAS  Google Scholar 

  • Koski M (1999) Carbon:nitrogen ratios of Baltic Sea copepods–indication of mineral limitation? J Plankton Res 21(8):1565–1573

    Article  Google Scholar 

  • Koski M, Kuosa H (1999) The effect of temperature, food concentration and female size on the egg production of the planktonic copepod Acartia bifilosa. J Plankton Res 21(9):1779–1789

    Article  Google Scholar 

  • Koski M, Schmidt K, Engström-Öst J, Viitasalo M, Jónasdóttir S, Repka S, Sivonen K (2002) Calanoid copepods feed and produce eggs in the presence of toxic cyanobacteria Nodularia spumigena. Limnol Oceanogr 47(3):878–885

    Article  Google Scholar 

  • Koueta N, Castro BG, Boucaud-Camou E (2000) Biochemical indices for instantaneous growth estimation in young cephalopod Sepia officinalis L. ICES J Mar Sci 57:1–7

    Article  Google Scholar 

  • Kyle M, Achaya K, Weider LJ, Looper K, Elser JJ (2006) Coupling growth rate and body stoichiometry in Daphnia: a role for maintenance processes? Freshw Biol 51:2087–2095

    Article  CAS  Google Scholar 

  • Lee R F, Hagen W, Kattner G (2006) Lipid storage in marine zooplankton. Mar Ecol Prog Ser 307:273–306

    Article  CAS  Google Scholar 

  • Ljunggren L, Sandström A, Johansson G, Sundblad G, Karås P (2005) Rekryteringsproblem hos Östersjöns kustfiskbestånd. Swedish Board of Fisheries, Finfo 2005:5 (in Swedish with summary in English)

  • MacKenzie BR, Alheit J, Conley DJ, Holm P, Kinze CC (2002) Ecological hypotheses for a historical reconstruction of upper trophic level biomass in the Baltic Sea and Skagerrak. Can J Fish Aquat Sci 59(1):173–190

    Article  Google Scholar 

  • Mercaldo-Allen R, Kuropat C, Caldarone E M (2006) A model to estimate growth in young-of-the-year tautog, Tautoga onitis, based on RNA/DNA ratio and seawater temperature. J Exp Mar Biol Ecol 329(2):187–195

    Article  CAS  Google Scholar 

  • Möllmann C, Köster FW (2002) Population dynamics of calanoid copepods and the implications of their predation by clupeid fish in the Central Baltic Sea. J Plankton Res 24(10):959–977

    Article  Google Scholar 

  • Möllmann C, Kornilovs G, Sidrevics L (2000) Long-term dynamics of main mesozooplankton species in the central Baltic Sea. J Plankton Res 22(11):2015–2038

    Article  Google Scholar 

  • Nakata K, Nakano H, Kikuchi H (1994) Relationship between egg productivity and RNA/DNA ratio in Paracalanus sp. in the frontal waters of the Kurshio. Mar Biol 119:591–596

    Article  Google Scholar 

  • Ojaveer H, Lankov A, Eero M, Kotta J, Kotta I, Lumberg A (1999) Changes in the ecosystem of the Gulf of Riga from the 1970s to the 1990s. ICES J Mar Sci 56(suppl.):33–40

    Article  Google Scholar 

  • Reinikanen M, Lindvall F, Meriluoto JAO, Repka S, Sivonen K, Spoof L, Wahlsten M (2002) Effects of dissolved cyanobacterial toxins on the survival and egg hatching of estuarine calanoid copepods. Mar Biol 140:577–583

    Article  Google Scholar 

  • Rosa R, Nunes ML (2003) Seasonal changes in nucleic acids, amino acids and protein content in juvenile Norway lobster (Nephrops norvegicus). Mar Biol 143:565–572

    Article  CAS  Google Scholar 

  • Rosa R, Nunes ML (2004) RNA, DNA and protein concentrations and amino acid profiles of deep-sea decapod Aristeus antennatus: an indication for seasonal variations of nutrition and growth. Aquat Living Resour 17:25–30

    Article  CAS  Google Scholar 

  • Runge JA, Roff JC (2000) The measurement of growth and reproductive rates. In: Harris R, Wiebe P, Lenz J, Skjoldal HR, Huntley M (eds). ICES zooplankton methodology manual, Academic, London, pp. 401–454

    Chapter  Google Scholar 

  • Rönkkönen S, Ojaveer E, Raid T, Viitasalo M (2004) Long-term changes in Baltic herring (Clupea harengus membras) growth in the Gulf of Finland. Can J Fish Aquat Sci 61:219–229

    Article  Google Scholar 

  • Saiz E, Calbet A, Fara A, Berdalet E (1998) RNA content of copepods as a tool for determining adult growth rates in the field. Limnol Oceanogr 43(3):465–470

    Article  CAS  Google Scholar 

  • Sidrevics L (1980) Investigations on the ecological characteristics of the main zooplankton species in the Central Baltic. In: Fisheries investigations in the Basin of the Baltic Sea. Riga, Avots 15:65–70 (in Russian)

  • Suneetha K-B, Folkvord A, Johannessen A (1999) Responsiveness of selected condition measures of herring, Clupea harengus, larvae to starvation in relation to ontogeny and temperature. Environ Biol Fish 54:191–204

    Article  Google Scholar 

  • Suresh AV, Sheehan RJ (1998) Biochemical and morphological correlates of growth in diploid and triploid rainbow trout. J Fish Biol 52:588–599

    Article  Google Scholar 

  • Suthers IM, Cleary JJ, Battaglene SC, Evans R (1996) Relative RNA content as a measure of condition in larval and juvenile fish. Mar Freshw Res 47(2):301–307

    Article  CAS  Google Scholar 

  • Tanskanen S (1994) Seasonal variability in the individual carbon content of the calanoid copepod Acartia bifilosa from the northern Baltic Sea. Hydrobiologia 292/293:397–403

    Article  Google Scholar 

  • Viitasalo M, Koski M, Pellikka K, Johansson S (1995) Seasonal and long-term variations in the body size of planktonic copepods in the northern Baltic Sea. Mar Biol 123:241–250

    Article  Google Scholar 

  • Vrede T, Persson J, Aronsen G (2002) The influence of food quality (P:C ratio) on RNA:DNA ratio and somatic growth rate of Daphnia. Limnol Oceanogr 47(2):487–494

    Article  CAS  Google Scholar 

  • Wagner M, Durbin E, Buckley L (1998) RNA:DNA ratios as indicators of nutritional condition in the copepod Calanus finmarchius. Mar Ecol Prog Ser 162:173–181

    Article  CAS  Google Scholar 

  • Wagner MM, Campbell RG, Boudreau CA, Durbin EG (2001) Nucleic acids and growth of Calanus finmarchius in the laboratory under different food and temperature conditions. Mar Ecol Prog Ser 221:185–197

    Article  CAS  Google Scholar 

  • Winberg GG, Patalas K, Wright JC, Hillbricht-Ilkowska A, Cooper WE, Mann KH (1971) Methods for calculating productivity. In: Edmondson WT, Winberg GG (eds) A manual on methods for the assessment of secondary productivity in fresh waters (IBP Handbook No 17), Adlard and son Ltd, Bartholomew press, Dorking, pp 296–317

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Acknowledgments

We thank J.-O. Persson (Mathematical Statistics, Stockholm University) for assistance with regression analysis and staff and colleagues at Tvärminne Zoological Station (Finland) and Askö Field Station (Sweden) for various help and support. We acknowledge also valuable comments from two anonymous reviewers on an earlier version of the manuscript. This study was financially supported by Walter and Andrée de Nottbeck Foundation (Finland) and Stockholm Marine Research Centre (Sweden).

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  1. Department of Systems Ecology, Stockholm University, 106 91, Stockholm, Sweden

    Towe Holmborn & Elena Gorokhova

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  1. Towe Holmborn
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  2. Elena Gorokhova
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Correspondence to Towe Holmborn.

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Communicated by M. Kühl.

An erratum to this article is available at http://dx.doi.org/10.1007/s00227-007-0887-6.

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Holmborn, T., Gorokhova, E. Relationships between RNA content and egg production rate in Acartia bifilosa (Copepoda, Calanoida) of different spatial and temporal origin. Mar Biol 153, 483–491 (2008). https://doi.org/10.1007/s00227-007-0838-2

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