Abstract
Species identification in the phylum Nematoda is complicated due to the paucity of easily obtainable diagnostic morphological features. Furthermore, the cosmopolitan distribution of several species despite low dispersal abilities makes cryptic diversity potentially substantial within this phylum. We conducted a population genetic survey in the marine nematode Geomonhystera disjuncta in Belgium and The Netherlands in two seasons. The mitochondrial cytochrome oxidase c subunit 1 (COI) gene was screened with the single-strand conformation polymorphism method in 759 individuals. The 43 haplotypes were grouped into five lineages, with low divergences within (<3%) and high divergences between lineages (>14%). Analysis of the nuclear ITS region yielded concordant tree topologies, indicating the presence of five cryptic taxa within G. disjuncta. Analysis of Molecular Variance (AMOVA) illustrated a significant structuring in all lineages and temporal fluctuations in haplotype frequencies within and between locations. Metapopulation dynamics and/or priority effects best explained this structuring. Finally, our data indicate that the COI gene may be useful for DNA barcoding purposes.
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Acknowledgments
The authors thank Dr. T. Remerie for his suggestions and help in retrieving the data and Dr. M. Raes for his help with the morphological measurements of the specimens. S.D. acknowledges a grant from the Flemish Institute for the Promotion of Scientific-Technological Research (I.W.T.). T.M. is a postdoctoral fellow with the Flemish Fund for Scientific Research. Further financial support was obtained from Ghent University in BOF-projects 1205398 (GOA) and 01GZ0705 (GOA-BBSea).
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Derycke, S., Backeljau, T., Vlaeminck, C. et al. Spatiotemporal analysis of population genetic structure in Geomonhystera disjuncta (Nematoda, Monhysteridae) reveals high levels of molecular diversity. Mar Biol 151, 1799–1812 (2007). https://doi.org/10.1007/s00227-007-0609-0
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DOI: https://doi.org/10.1007/s00227-007-0609-0